高等学校化学学报

高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (5): 671.

• 论文 • 上一篇    下一篇

阿霉素─铁(Ⅲ)配合物的电化学特征及其与DNA结合作用研究

王宏飞, 杨频, 李青山, 邬强   

  1. 南京大学化学系, 配位化学国家重点实验室, 山西大学分子科学研究所, 太原, 030006
  • 收稿日期:1996-04-16 出版日期:1997-05-24 发布日期:1997-05-24
  • 通讯作者: 杨频.
  • 作者简介:王宏飞,男,27岁,博士研究生.
  • 基金资助:

    国家自然科学基金;山西省自然科学基金

Electrochemistry Characteristics of Adriamycin-Fe(Ⅲ) Complex and Its Interaction with DNA

WANG Hong-Fei, YANG Pin, LI Qing-Shan, WU Qiang   

  1. Department of Chemistry, Nanjing University, State Key Lab.of Coordination Chemistry, Nanjing, 210093, Institute of Molecular Science, Shanxi University, Taiyuan, 030006
  • Received:1996-04-16 Online:1997-05-24 Published:1997-05-24

PDF (PC)

被引次数

30

摘要： 采用示波极谱法和循环伏安法研究了3公铁离子Fe(Ⅲ)与阿霉素(ADM)配合物的电化学特性.在生理pH条件下,Fe(Ⅲ)与ADM形成2：1的稳定配合物,Fe(Ⅲ)的配位使ADM在更负的电势下才能还原,这与其心脏毒性减轻密切相关.采用线性扫描、吸收光谱和凝胶电泳法研究了ADM－Fe(Ⅲ)配合物与DNA的结合作用,ADM－Fe(Ⅲ)配合物仍保留了ADM插入结合的特征,并形成一种稳定的DNA－Fe(Ⅲ)－ADM的三元结合物.

关键词: 阿霉素, 3价铁离子配合物, 心脏毒性, DNA损伤

Abstract: Electrochemistry characteristics of adriamycin-Fe(Ⅲ) complex was studied by the methods of osillopolarograph and cyclic voltammetry.At physiological pH, Fe (Ⅲ) binds with ADMto form a complex of 2: 1 stoichiometry.ADMwas reduced at a more negative potential when it was complexed with Fe(Ⅲ), which is close related to the low cardiac toxicity of the complex.The interaction model of ADM-Fe(Ⅲ) complex with DNAwas studied by the methods of spectroscopy, electrochemlstry and electrophoresis.The complex preserves the DNAintercalation characteristics, and binds to DNAto form a ternary complex composed of DNA, Fe(Ⅲ) and ADM.

Key words: Adriamycin, Fe(Ⅲ) complex, Cardiac toxicity, DNA damage

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