高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (2): 245.

• 论文 • 上一篇    下一篇

Nafion/Au溶胶自组装微铂传感器的研究及其在心肌细胞中NO水平测定中的应用

朱民, 刘敏, 施国跃, 陈俊水, 金利通   

  1. 华东师范大学化学系, 上海 200062
  • 收稿日期:2001-08-28 出版日期:2003-02-24 发布日期:2003-02-24
  • 通讯作者: 金利通(1937年出生),男,博士生导师,主要从事生物电分析方面的研究.E-mial:litongjin@263.net
  • 基金资助:

    上海市青年科技启明星计划(批准号:00QA14043);中国科学院长春应用化学研究所电分析化学国家重点实验室资助

A Novel NO Microsensor and Its Application in the Detection of NO in Myocardial Cells

ZHU Min, LIU Min, SHI Guo-Yue, CHEN Jun-Shui, JIN Li-Tong   

  1. Department of Chemistry, East China Normal University, Shanghai 200062, China
  • Received:2001-08-28 Online:2003-02-24 Published:2003-02-24

PDF (PC)

被引次数

25

摘要： 制备了一种新型的Nafion/Au溶胶修饰微铂传感器(Au溶胶颗粒的直径为7～14nm),并将该传感器应用于心肌细胞中NO水平的研究.实验结果表明,自组装制备的Nafion/Au溶胶修饰微传感器对NO有较高的灵敏度和良好的选择性,在1.0×10-7～4.0×10-5mol/L浓度范围内,NO的催化氧化电流与其浓度呈良好线性关系,检测限为5.0×10-8mol/L.探讨了该修饰微传感器对NO的催化机理,研究了在L-精氨酸和乙酰胆碱刺激下心肌细胞内的NO释放.

关键词: 金溶胶, 微传感器, 心肌细胞, 一氧化氮

Abstract: A novel sensitive, selective and stable nitric oxide(NO) microsensor is described, which is modified by nano Au colloid and Nafion. Determined by Atomic Forced Microscope, the diameter of Au colloid particles is from 7 to 14 nanometers. The detection of NO is based on the nano Au particles catalysis of NO oxidation at an anodic potential of +0.74 V(vs. SCE). The microsensor showed a low detection limit, high selectivity and sensitivity for NO determination. The oxidation current(measured by differential pulse amperometric technique) was linear with NO concentration ranging from 1.0×10-7 to 4.0×10-5 mol/L with a calculated detection limit of 5.0×10-8 mol/L(S/N=3). Using the microsensor, the direct, real time production of NO in the myocardial cells was continuously measured, which showed the NO levels was increased by stimulating with L-arginine and acetylcholine.

Key words: Au colloid, Microsensor, Myocardial cells, Nitric oxide

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