高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (1): 61.

• 研究论文 • 上一篇    下一篇

酶解猪血蛋白中活性肽的纯化和功能研究

李艳伟1, 江波2, 佟祥山1   

  1. 1. 吉林大学生命科学学院,长春 130023;
    2. 长春生物制品研究所,长春 130021
  • 收稿日期:2003-12-30 出版日期:2005-01-10 发布日期:2005-01-10
  • 通讯作者: 佟祥山(1947年出生),男,教授,主要从事生物化学制药方面的研究.E-mail:shengkeyuan.student@sina.com E-mail:shengkeyuan.student@sina.com

Purification and Function Research of Active Peptide from Pig Blood Hydrolyzed by Trypsin

LI Yan-Wei1, JIANG-Bo2, TONG Xiang-Shan1   

  1. 1. College of Life Science, Jilin University, Changchun 130023, China;
    2. Changchun Institute of Biological Products, Changchun 130021, China
  • Received:2003-12-30 Online:2005-01-10 Published:2005-01-10

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被引次数

84

摘要： 利用层析、超滤等生物化学手段纯化猪血胰蛋白酶水解液,得到的目标产物是一种在高压液相色谱水平上的纯品,分子量大约为3000的小肽.利用T淋巴细胞转化实验和E花环形成实验确定目标小肽的存在,并分别采用体内、体外的免疫学和药理学实验,初步确定了目标小肽对机体免疫活性的增强性质.

关键词: 生物活性肽, T淋巴细胞转化实验, 猪血胰蛋白酶

Abstract: To purify the enzymolysis product of porcine blood by trypsin hydrolysis via the biological technique such as ultra-filter and chromatography, we obtain the aim product using HPLC to determine the purity of our aim peptide, its molecular weight is about 3 000. In order to identify the existence of our aim peptide, we performed T lymophocyte transformation test and E rosette forming test for the product of the progress. Furthermore, we carried out the experiments of immunology and pharmacology in vivo and in vitro to ensure that our aim peptide can prove the enhancement immunologic activity of organism.

Key words: Bioactive peptide, T lymphocyte transformation test, Pig blood hydrolyzed by trypsin

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