高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

Ce-Cur纳米酶对巨噬细胞极化的调控研究

许海燕，刘琦，孟和，刘风琦，刘雨昕，李嘉伟，李常艳   

  1. 内蒙古大学化学化工学院
  • 收稿日期:2025-07-07 修回日期:2025-10-11 网络首发:2025-10-15 发布日期:2025-10-15
  • 通讯作者: 李常艳 E-mail:celicy@imu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号：22067015)、内蒙古2024年度留学人员创新创业启动项目(批准号：12000-15072405)和内蒙古自治区研究生精品课程项目(批准号：JP20231008)资助

Study on the Regulation of Macrophage Polarization by Ce-Cur Nanoenzymes

XU Haiyan，LIU Qi，MENG He，LIU Fengqi，LIU Yuxin，LI Jiawei，LI Changyan   

  1. College of Chemistry and Chemical Engineering, Inner Mongolia University
  • Received:2025-07-07 Revised:2025-10-11 Online First:2025-10-15 Published:2025-10-15
  • Contact: Chang-Yan Li E-mail:celicy@imu.edu.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China (No.22067015), the Innovation and Entrepreneurship Start-up Project for overseas students in 2024 Inner Mongolia Autonomous Region (No.12000-15072405) and the project of high-quality graduate courses in Inner Mongolia Autonomous Region (No.JP20231008)

PDF (PC)

摘要： 本文以氯化铈(CeCl3)和姜黄素(Cur)为原料，以聚乙烯吡咯烷酮(PVP)为分散剂，设计并合成了无机-有机复合型Ce-Cur纳米酶. 采用X射线粉末衍射(XRD)、透射电子显微镜(TEM)、X射线光电子能谱(XPS)、傅里叶变换红外光谱(FT-IR)、Zeta电位及粒度分析等手段对其微观结构和表面性质进行了系统表征. 通过ABTS和DPPH自由基清除实验评价了该纳米酶的体外抗氧化活性，并利用DCFH-DA荧光探针法检测了其对巨噬细胞内活性氧物种(ROS)的清除能力，结果表明Ce-Cur纳米酶具有良好的抗氧化能力，可显著降低细胞内ROS水平(荧光强度 MFI 下降53%). CCK8 法与死活荧光染色实验显示该纳米酶具有良好的细胞相容性. 实时荧光定量PCR(RT-qPCR)、免疫荧光及ELISA分析数据显示，Ce-Cur纳米酶能够有效抑制由LPS+IFN-γ诱导的炎症反应，下调M1型炎症因子TNF-α、IL-1β和IL-6的mRNA表达与蛋白分泌(M1抑制率分别为57%、67%和82%)，同时促进M2型抗炎因子IL-10的表达(M2 提升率达351%). 上述结果表明，姜黄素与无定形氧化铈组分之间存在协同抗氧化与抗炎效应，可显著调控巨噬细胞由M1向M2表型极化. 本研究为Ce-Cur纳米酶在免疫调节及相关炎症疾病治疗领域的应用提供了实验依据与潜在策略.

关键词: Ce-Cur纳米酶, 巨噬细胞, M1促炎因子, M2抗炎因子, 极化作用

Abstract: This study designed and synthesized an inorganic-organic composite Ce-Cur nanoenzyme using cerium chloride (CeCl3) and curcumin (Cur) as raw materials, with polyvinylpyrrolidone (PVP) as the dispersant. Systematic characterization of its microstructure and surface properties was performed using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), zeta potential, and particle size analysis. In vitro antioxidant activity was evaluated via ABTS and DPPH radical scavenging assays, while DCFH-DA fluorescence probe-based assays assessed its ability to scavenge reactive oxygen species (ROS) within macrophages. Results indicate that the Ce-Cur nanozyme exhibits excellent antioxidant capacity, significantly reducing intracellular ROS levels (fluorescence intensity MFI decreased by 53%). CCK8 assays and live/dead fluorescent staining experiments demonstrated excellent cellular compatibility of the nanozyme. Real-time quantitative PCR (RT-qPCR), immunofluorescence, and ELISA analyses revealed that Ce-Cur nanozyme effectively suppressed LPS+IFN-γ-induced inflammatory responses. It downregulated mRNA expression and protein secretion of M1-type inflammatory cytokines TNF-α, IL-1β, and IL-6 (The M1 inhibition rates were 57%, 67%, and 82%, respectively.), while simultaneously promoting the expression of the M2 anti-inflammatory factor IL-10 (M2 growth rate reached 351%). These results indicate synergistic antioxidant and anti-inflammatory effects between curcumin and amorphous cerium oxide components, significantly regulating macrophage polarization from the M1 to the M2 phenotype. This study provides experimental evidence and potential strategies for the application of Ce-Cur nanoenzymes in immunomodulation and the treatment of related inflammatory diseases.

Key words:  Ce-Cur nanoenzymes；Macrophage；M1-type inflammatory factors；M2-type anti-inflammatory factors, Polarization

中图分类号: 

TrendMD: