高等学校化学学报

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (9): 1739.

• 研究论文 • 上一篇    下一篇

当归多糖ASP3及其水解产物的NMR光谱分析

孙元琳1, 陕方2, 崔武卫3, 汤坚4, 顾小红4   

  1. 1. 运城学院生命科学系, 运城 044000;
    2. 山西省农科院农产品综合利用研究所, 太原 030031;
    3. 加拿大农业部食品研究所, 安大略省 N1G5C9;
    4. 江南大学食品科学与安全教育部重点实验室, 无锡 214036
  • 收稿日期:2009-03-04 出版日期:2009-09-10 发布日期:2009-09-10
  • 通讯作者: 孙元琳, 女, 博士, 副教授, 主要从事生物大分子的结构与功能研究. E-mail: sylwts@yahoo.com.cn
  • 基金资助:

    山西省青年科研基金(批准号: 2007021042)、院级重点科研基金(批准号: 20060102)和院级学术带头人基金资助.

NMR Analysis of Polysaccharide ASP3 from Angelica sinensis(Oliv.) Diels and It′s Hydrolysis Products

SUN Yuan-Lin1*, SHAN Fang2, CUI Wu-Wei3, TANG Jian4, GU Xiao-Hong4   

  1. 1. Department of Life Science, Yuncheng University, Yuncheng 044000, China;
    2. Institute of Farm Products Comprehensive Utilization, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;
    3. Food Research Program, Agriculture and Agri-Food, Ontario N1G 5C9, Canada;
    4. Key Laboratory of Food Science and Safety, Ministry of Education, Jiangnan University , Wuxi 214036, China
  • Received:2009-03-04 Online:2009-09-10 Published:2009-09-10
  • Contact: SUN Yuan-Lin. E-mail: sylwts@yahoo.com.cn
  • Supported by:

    山西省青年科研基金(批准号: 2007021042)、院级重点科研基金(批准号: 20060102)和院级学术带头人基金资助.

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被引次数

40

摘要：

对当归多糖ASP3的糖链结构进行分析. 分别采用0.2 mol/L三氟乙酸(Trifluoroacetic acid, TFA)和内切-α-(1→4)-聚半乳糖醛酸酶(EndoPG)对ASP3进行部分酸水解和酶水解, 并对水解前后多糖组分的1D和2D NMR光谱特征进行分析. 实验结果表明, ASP3是一种果胶多糖. GalpA和Rhap位于多糖分子的主链, 由1→4-D-GalpA相连形成的“光滑区”(半乳糖醛酸聚糖)是其主要组成部分; 由α-(1→4)-GalpA通过O4位与α-(1→2)-和α-(1→2,4)-Rhap的O2位交替连接所形成的重复单元[→4)-α-GalpA-(1→2)-α-Rhap-(1→]构成具有较高分支的“毛发区”(富含中性糖侧链的鼠李半乳糖醛酸聚糖). Galp和Araf是中性糖侧链的主要组成, 通过Rhap残基的O4位与主链相连. 非还原性末端T-β-Galp, β-(1→3)-, β-(1→3,6)-, β-(1→4)-, β-(1→4,6)-Galp聚合形成以β-(1→3,6)-Galp为分支点的β-(1→6)-半乳聚糖和以β-(1→4,6)-Galp为分支点的β-(1→4)-半乳聚糖. T-α-Araf, α-(1→5)-Araf和α-(1→3,5)-Araf聚合形成以α-(1→3,5)-Araf为分支点的α-(1→5)-阿拉伯聚糖. 此外, 由α-(1→5)-阿拉伯聚糖通过α-(1→3)连接与β-(1→6)-半乳聚糖末端聚合形成阿拉伯半乳聚糖.

关键词: 当归; 果胶多糖; 部分酸水解; 酶水解; 核磁共振

Abstract:

The structural features of polysaccharide ASP3 were studied. ASP3 was hydrolyzed with 0.2 mol/L trifluoroacetic acid and was enzymic hydrolyzed with endo-α-(1→4)-polygalacturonases. NMR analysis method was conducted on the samples. The results indicated that ASP3 was a kind of pectic polysaccharide which contained the main part of homogalacturonan fragments as “smooth regions” with α-(1→4)-GalpA, and rhamnogalacturonan segments as “hairy regions” backbone. Gal and Ara, which were the mainly constitution of the branch, substituted at O4 position of Rhap residues in the backbone. Man and Glc were linked as arabinogalactan, galactan and arabinan. Terminal, β-(1→3)-, β-(1→3,6)-, β-(1→4)-, and β-(1→4,6)-Galp as well as residues of terminal, α-(1→5)-, and α-(1→3,5)-Araf were linked to form β-(1→6)- or β-(1→4)-galactans bearing 3,6- or 4,6-substituted β-Galp residues as branched points respectively, and short α-(1→5)-arabinan possessing 3,5-substituted α-Ara residues as branched points. In addition, arabinogalactan was found to contain branched sugar chains of α-(1→5)-arabinan attached to the linear chains of β-(1→6)-galactan by 1,3-linkages.

Key words: Angelica sinensis; Pectic polysaccharide; Partial acid hydrolysis; Enzymic digestion; NMR

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