磁芯负载离子液体凝胶微球的制备、表征及在固定化细胞技术中的应用

doi:10.7503/cjcu20180660

高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (4): 793.doi: 10.7503/cjcu20180660

磁芯负载离子液体凝胶微球的制备、表征及在固定化细胞技术中的应用

黄晓林^1,², 谢睆^1,³, 曹红¹(), 金宏杰^1,⁴, 李春⁵, 吴廷华²

1. 嘉兴学院生物与化学工程学院, 嘉兴 314001
2. 浙江师范大学物理化学研究所, 金华 321004
3. 新疆医科大学药学院, 乌鲁木齐830011
4. 石河子大学生命科学学院, 石河子 832003
5. 北京理工大学生命科学与技术学院, 北京 100081

收稿日期:2018-09-29 出版日期:2019-04-03 发布日期:2019-01-24
作者简介:
联系人简介: 曹红, 女, 博士, 研究员, 主要从事生物催化与酶工程方面的研究. E-mail: chyf_ch@126.com
基金资助:
国家自然科学基金(批准号: 21266029)和浙江省自然科学基金(批准号: LY17B060011)资助.

Preparation, Characterization of Magnetic Core Gel Microspheres Loaded Ionic Liquids and Its Application in Cells Immobilization Technique^†

HUANG Xiaolin^1,², XIE Huan^1,³, CAO Hong^1,^*(), JIN Hongjie^1,⁴, LI Chun⁵, WU Tinghua²

1. College of Biological Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, China
2. Institute of Physical Chemistry, Zhejiang Normal University, Jinhua 321004, China
3. College of Pharmacy, Xinjiang Medical University, Urumqi 830011, China
4. College of Life Sciences, Shihezi University, Shihezi 8320034, China
5. School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China

Received:2018-09-29 Online:2019-04-03 Published:2019-01-24
Contact: CAO Hong E-mail:chyf_ch@126.com
Supported by:
† Supported by the National Natural Science Foundation of China(No. 21266029) and the Natural Science Foundation of Zhejiang Province, China(No.LY17B060011).

摘要/Abstract

摘要：

选取具有良好生物相容性的壳聚糖(CS)包覆四氧化三铁纳米粒子(Fe₃O₄/CS)作为磁响应材料, 制备了磁芯负载1-丁基-3-甲基咪唑六氟磷酸盐([BMIM]PF₆)凝胶微球; 对Fe₃O₄/CS及磁芯负载离子液体凝胶微球的组成、结构、微观形貌和磁性能进行了表征; 将其应用于固定化细胞技术, 在产紫青霉细胞全细胞生物催化甘草酸(GL)合成单葡萄糖醛酸基甘草次酸(GAMG)体系中, 实现了对全细胞生物催化剂和离子液体的快速回收和重复利用. 实验结果表明, 壳聚糖成功包裹Fe₃O₄纳米粒子; Fe₃O₄/CS均匀分布在凝胶微球内部, 并显示出良好的磁性能; 与凝胶微球固定化细胞催化体系相比, 磁芯负载[BMIM]PF₆凝胶微球固定化细胞催化体系中GAMG的产率提高了13.8%; 重复利用实验结果表明, 磁芯负载[BMIM]PF₆凝胶微球固定化产紫青霉细胞在外加磁场的作用下, 易于快速回收, 并且循环再利用9次后相对活性仍保留59.2%.

关键词: Fe₃O₄/CS纳米粒子, 磁芯负载离子液体凝胶微球, 1-丁基-3-甲基咪唑六氟磷酸盐, 固定化细胞技术, 生物催化

Abstract:

The magnetic core gel microspheres loaded ionic liquids(ILs) were prepared using Fe₃O₄ nanoparticles coated with chitosan with good biocompatibility(Fe₃O₄/CS) as magnetic response material. The composition, structure, morphology and magnetic properties of the prepared Fe₃O₄/CS nanoparticles and magnetic core gel microspheres loaded [BMIM]PF₆ were characterized by Fourier transform infrared spectroscopy(FTIR), scanning electron microscope(SEM), transmission electron microscope(TEM), X-ray diffraction(XRD), thermogravimetric analysis(TG) and vibrating sample magnetometer(VSM). And the magnetic core gel microspheres loaded [BMIM]PF₆ was applied to immobilize Penicillium purpurogenum Li-3 cells for the biosynthesis of glycyrrhetinic acid monoglucuronide(GAMG) from glycyrrhizic acid(GL) to realize the rapid recovery and reuse of the whole-cell biocatalyst and ILs. The results showed that the Fe₃O₄ nanoparticles were successfully encapsulated by chitosan. And due to the uniform distribution of magnetic particles in the prepared gel microspheres loaded ILs, the microspheres show good magnetic properties. Compared with immobilized cells with gel microsphere(Ic) catalytic system, the yield of GAMG in immobilized cells with magnetic core gel microspheres loaded [BMIM]PF₆(IcMILs) catalytic system increased by 13.8%. The reutilization experiment showed that the IcMILs were easier to be recovered quickly under the applied magnetic field. After 9 cycles of recycling, the IcMILs relative activity still remained 59.2%. The preparation technique of the magnetic core gel microspheres loaded ILs has the potential to be further developed and applied in the rapid on-line separation and continuous operation of biocatalyst and ILs to increase the catalytic yield and reduce the cost.

Key words: Fe₃O₄/CS nanoparticles, Magnetic core gel microspheres loaded ionic liquid, [BMIM]PF₆, Cells immobilization technique, Biocatalysis

中图分类号:

O643.3

TrendMD:

黄晓林, 谢睆, 曹红, 金宏杰, 李春, 吴廷华. 磁芯负载离子液体凝胶微球的制备、表征及在固定化细胞技术中的应用. 高等学校化学学报, 2019, 40(4): 793.

HUANG Xiaolin,XIE Huan,CAO Hong,JIN Hongjie,LI Chun,WU Tinghua. Preparation, Characterization of Magnetic Core Gel Microspheres Loaded Ionic Liquids and Its Application in Cells Immobilization Technique^†. Chem. J. Chinese Universities, 2019, 40(4): 793.

图/表 8

Fig.1 TEM images of Fe3O4(A) and Fe3O4/CS(B)

Fig.2 SEM images of magnetic gel beads(A) and magnetic supported ionic liquid [BMIM]PF6 gel beads(B—F)Volume fraction of [BMIM]PF6: (A) 0; (B) 3%; (C) 6%; (D) 9%; (E) 12%; (F) 15%.

Fig.3 FTIR spectra of the chitosan(a), Fe3O4/CS(b), Fe3O4(c), magnetic supported [BMIM]PF6 gel beads(d) and [BMIM]PF6(e)

Fig.4 TG curves of Fe3O4(a), Fe3O4/CS(b) and magnetic supported [BMIM]PF6 gel beads(c)

Fig.5 XRD patterns of Fe3O4(a), Fe3O4/CS(b) and magnetic gel beads(c)

Fig.6 VSM of Fe3O4(a), Fe3O4/CS(b) and magnetic gel beads(c)

Fig.7 Yields of GAMG catalysed by Ic(a), IcMILs(b) and free cell(c)

Fig.8 Repeated utilization of magnetic immobilized cells supported with [BMIM]PF6

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磁芯负载离子液体凝胶微球的制备、表征及在固定化细胞技术中的应用

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磁芯负载离子液体凝胶微球的制备、表征及在固定化细胞技术中的应用

Preparation, Characterization of Magnetic Core Gel Microspheres Loaded Ionic Liquids and Its Application in Cells Immobilization Technique†

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Preparation, Characterization of Magnetic Core Gel Microspheres Loaded Ionic Liquids and Its Application in Cells Immobilization Technique^†