海带褐藻多糖硫酸酯的降解与岩藻寡糖的制备

doi:10.7503/cjcu20170232

高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (12): 2193.doi: 10.7503/cjcu20170232

海带褐藻多糖硫酸酯的降解与岩藻寡糖的制备

耿丽华^1,^2,³, 金维华¹, 王晶^1,³, 张全斌^1,³()

1. 中国科学院海洋研究所实验海洋生物学重点实验室, 青岛 266071
2. 中国科学院大学, 北京 100049
3. 青岛海洋科学与技术国家实验室, 海洋生物学与生物技术功能实验室, 青岛 266071

收稿日期:2017-04-13 出版日期:2017-12-10 发布日期:2017-11-20
作者简介:联系人简介: 张全斌, 男, 博士, 研究员, 博士生导师, 主要从事海藻化学与海洋药物研究. E-mail: qbzhang@qdio.ac.cn
基金资助:
国家自然科学基金(批准号: 41506165)、山东省重点研发计划项目(海洋医用食品)(批准号: 2016YYSP002)、山东省自然科学基金(批准号: ZR2014DQ024)和青岛市应用基础研究计划-青年专项(批准号: 15-9-1-74-jch)资助

Fucoidan Degradation and Preparation of Fuco-oligosaccharides from Saccharina japonica^†

GENG Lihua^1,^2,³, JIN Weihua¹, WANG Jing^1,², ZHANG Quanbin^1,^3,^*()

1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences,Qingdao 266071, China
2. University of Chinese Academy of Sciences, Beijing 100049, China
3. Lab for Marine Biology and Biotechnology, Qingdao National Lab for Marine Science and Technology,Qingdao 266071, China

Received:2017-04-13 Online:2017-12-10 Published:2017-11-20
Contact: ZHANG Quanbin E-mail:qbzhang@qdio.ac.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.41506165), the Focus on Research and Development Plan in Shandong Province(Marine Medical Food), China(No.2016YYSP002), the Shandong Provincial Natural Science Foundation of China(No.ZR2014DQ024) and the Science and Technology Program of Applied Basic Research Projects of Qingdao Municipal, China(No.15-9-1-74-jch)

摘要/Abstract

摘要：

采用稀酸水解的方法降解粗褐藻多糖硫酸酯(FPS)制备了硫酸化岩藻寡糖. 通过考察降解及沉降条件研究了FPS的水解及沉降规律. 通过理化性质分析发现, 沉降所得上层清液为杂合硫酸化岩藻寡糖[岩藻糖(Fuc)含量>55%]. 进一步采用Bio-Gel P4低压凝胶渗透色谱分离纯化, 得到4种以岩藻糖为主的低聚合度硫酸化寡糖. 单糖组成分析和电喷雾质谱(ESI-MS)分析结果表明, 各组分主要为系列低聚合度硫酸化岩藻寡糖或硫酸化岩藻糖, 包括Fuc_1~3S_1~3, Fuc_1~2S_1~2, Fuc_1~2S₁及FucGlcAS.

关键词: 褐藻多糖硫酸酯, 岩藻寡糖, 酸水解

Abstract:

The sulfated fuco-oligosaccharides were prepared via acid hydrolysis degradation of crude fucoidan extracted from Saccharina japonica. Through the optimization of degradation and precipitation conditions, the hydrolysis and sedimentation law of fucoidan were studied. Physicochemical analysis showed that the supernatant was heterozygous sulfated fuco-oligosaccharide(fucose>55%). Four fractions of fucose based low polyme-rization degree of oligosaccharides were purified by Bio-Gel P4 low-pressure permeation chromatography, and the fucose content of the fractions was up to 90% according to monosaccharide composition analysis and electrospray ionization mass spectrometry(ESI-MS) analysis. Thus, the fractions were series of low-polymerized sulfated fuco-oligosaccharide/fucose, and the major fractions could be expressed with the formula of Fuc_1~3S_1~3, Fuc_1~2S_1~2, Fuc_1~2S, and FucGlcAS.

Key words: Fucoidan, Fuco-oligosaccharide, Acid hydrolysis

中图分类号:

O629

TrendMD:

耿丽华, 金维华, 王晶, 张全斌. 海带褐藻多糖硫酸酯的降解与岩藻寡糖的制备. 高等学校化学学报, 2017, 38(12): 2193.

GENG Lihua, JIN Weihua, WANG Jing, ZHANG Quanbin. Fucoidan Degradation and Preparation of Fuco-oligosaccharides from Saccharina japonica^†. Chem. J. Chinese Universities, 2017, 38(12): 2193.

图/表 4

Table 1 Condition setting of degradation of crude fucoidan from seaweed

Condition	Level
Condition	1	2	3
Hydrolysis time/h	3	5	7
Precipitation reagent	EtOH	AcN	(CH₃)₂CO
Concentration(%)	50	70	90

Fig.1 Monosaccharides composition of SPF1(mass ratio) a. 1-1-1; b. 1-2-1; c. 1-3-1; d. 2-1-1; e. 2-2-1; f. 2-3-1; g. 3-1-1; h. 3-2-1; i. 3-3-1; j. 1-1-2; k. 1-2-2; l. 1-3-2; m. 2-1-2; n. 2-2-2-; o. 2-3-2; p. 3-1-2; q. 3-2-2; r. 3-3-2; s. 1-1-3; t. 1-2-3; u. 1-3-3; v. 2-1-3; w. 2-2-3; x. 2-3-3; y. 3-1-3; z. 3-2-3; z. 3-3-3.

Fig.2 Separation graph(A) and TLC analysis for fractions FP1—FP4(B) of SPF1 by LPGPC (B) Std.: standard of fucose; lanes 1—4: FP1—FP4.

Table 2 Negative ion ESI-MS data of fucoidan-derived sulfated oligosaccharide fragments FP1—FP4

Fraction	Degree of polymerization	Structure	Found ion(H form)
Fraction	Degree of polymerization	Structure	[M-H]^-	[M-2H]^2-	[M-3H]^3-
FP1	1	FucS	243.0
	1	FucS₂	323.0	161.0
	2	FucGlcAS	419.2
	2	Fuc₂S	389.0
	2	Fuc₂S₂	469.0	234.0
	2	Fuc₂S₃	549.0	274.0	182.3
	3	Fuc₃S	535.1
	3	Fuc₃S₂	615.0	307.0
FP2	1	FucS	243.0
	1	FucS₂	323.0	161.0
	2	Fuc₂S	389.0
	2	Fuc₂S₂	469.0	234.0
FP3	1	FucS	243.0
	2	Fuc₂S	389.0
FP4	1	FucS	243.0

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海带褐藻多糖硫酸酯的降解与岩藻寡糖的制备

Fucoidan Degradation and Preparation of Fuco-oligosaccharides from Saccharina japonica^†

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海带褐藻多糖硫酸酯的降解与岩藻寡糖的制备

Fucoidan Degradation and Preparation of Fuco-oligosaccharides from Saccharina japonica†

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Fucoidan Degradation and Preparation of Fuco-oligosaccharides from Saccharina japonica^†