高等学校化学学报

高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (05): 964.doi: 10.3969/j.issn.0251-0790.2012.05.018

• 有机化学 • 上一篇    下一篇

黑小麦麦麸戊聚糖的部分酸水解特征与甲基化分析

孙元琳1, 顾小红2, 陕方3, 张俊明1, 崔武卫4   

  1. 1. 运城学院生命科学系, 运城 044000;
    2. 江南大学食品科学与技术国家重点实验室, 无锡 214122;
    3. 山西省农业科学院农产品综合利用研究所, 太原 030031;
    4. 加拿大农业部食品研究所, 安大略省 N1G5C9
  • 收稿日期:2011-07-12 出版日期:2012-05-10 发布日期:2012-05-10
  • 作者简介:孙元琳, 女, 博士, 副教授, 主要从事生物大分子的结构与功能研究. E-mail: sylwts@yahoo.com. cn
  • 基金资助:

    国家自然科学基金(批准号: 31101244)和山西省高等学校优秀青年学术带头人支持计划项目资助.

Partial Acid Hydrolytic Characteristics and Methylation Analysis of Pentosans from Black-grained Wheat Bran

SUN Yuan-Lin1, GU Xiao-Hong2, SHAN Fang3, ZHANG Jun-Ming1, CUI Wu-Wei4   

  1. 1. Department of Life Science, Yuncheng University, Yuncheng 044000, China;
    2. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
    3. Institute of Farm Products Comprehensive Utilization, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;
    4. Food Research Program, Agriculture and Agri-Food, Ontario N1G5C9, Canada
  • Received:2011-07-12 Online:2012-05-10 Published:2012-05-10

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被引次数

5

摘要： 研究了黑小麦麦麸戊聚糖(AEPH)的部分酸水解特征及其糖链的取代度和取代方式等结构信息. 分别采用0.02, 0.1和0.2 mol/L的三氟乙酸(Trifluoroacetic acid, TFA)对AEPH进行部分酸水解, 结合甲基化分析、 GC-MS和HPLC等方法对AEPH的糖链结构和相对分子质量分布进行了分析. 结果表明, 戊聚糖AEPH的糖残基主要由阿拉伯糖(Araf)和木糖(Xylp)组成. 其取代度(A/X), 即Araf与Xylp的比值为0.60, 并具有较高的相对分子质量(Mw=3.81×105). AEPH的主链由Xylp残基通过1→4连接形成木聚糖. 主链Xylp在O2, O3或O2/O3位被Araf单取代或双取代. 非取代(u)、 单取代(m)和双取代(d)Xylp残基的组成比例为57.7∶22.0∶6.2. 非取代与取代Xylp的比值为2.1, 双取代与单取代Xylp比值(d/m)为0.3. Araf主要以非还原性末端T-Araf以及通过1→2和1→5连接形成低聚阿拉伯糖的形式与主链Xylp相连, 或与T-Xylp相连形成戊聚糖分子的支链.

关键词: 黑小麦, 戊聚糖, 部分酸水解, 甲基化分析

Abstract: The hydrolytic characteristics and the structural features of substitute degree as well as substitute patterns of pentosans(AEPH) from black-grained wheat bran were analyzed. AEPH was hydrolyzed with 0.02, 0.1, and 0.2 mol/L trifluoroacetic acid(TFA), respectively. The structural features and Mw of AEPH were studied with methylation methods, combined with GC-MS and HPLC instruments. The results indicated that the residues of sugar constituents were mainly Araf and Xylp, with an Ara/Xyl(A/X) ratio of 0.60 and a high average molecular weight(Mw) of 3.81×105. AEPH contained a xylan backbone of 1→4 linked Xylp residues which was mono- or disubstituted with Araf groups at position O3, O2 or at both O2 and O3 positions. The relative composition ratio of un-, mono-, and di-substituted Xylp in the arabinoxylan fraction was 57.7∶22.0∶6.2, with a high proportion of unsubstituted to substituted Xylp(2.1), and a very low proportion of di- to monosubstituted Xylp residues(d/m, 0.3). Araf side groups were linked to Xylp residues of the backbone as terminal Araf single units. Moreover, small quantities of 1→2, and 1→5 linked Araf indicated the presence of short arabinan side chains terminated with T-Xylp residues.

Key words: Black-grained wheat, Pentosan, Partial acid hydrolysis, Methylation analysis

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