四氧化三铁纳米粒子表面接枝聚L-谷氨酸苄酯多孔微载体的构建和性能

doi:10.7503/cjcu20160701

高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (7): 1295.doi: 10.7503/cjcu20160701

• 高分子化学 • 上一篇

四氧化三铁纳米粒子表面接枝聚L-谷氨酸苄酯多孔微载体的构建和性能

徐盛华, 夏鹏飞, 张坤玺, 高龙, 尹静波()

上海大学材料科学与工程学院高分子材料系, 上海 200444

收稿日期:2016-10-08 出版日期:2017-07-10 发布日期:2017-05-25
作者简介:联系人简介: 尹静波, 女, 博士, 教授, 主要从事生物医用和生态环境高分子材料研究. E-mail: jbyin@oa.shu.edu.cn
基金资助:
上海市科委基础研究重点项目(批准号: 15JC1490400)和国家自然科学基金(批准号: 51373094)资助

Fabrication and Performance for Fe₃O₄ Nanoparticles Surface Grafted Poly(γ-benzyl-L-glutamate) Porous Microcarriers^†

XU Shenghua, XIA Pengfei, ZHANG Kunxi, GAO Long, YIN Jingbo^*()

Department of Polymer Materials, School of Materials Science and Engineering,Shanghai University, Shanghai 201800, China

Received:2016-10-08 Online:2017-07-10 Published:2017-05-25
Contact: YIN Jingbo E-mail:jbyin@oa.shu.edu.cn
Supported by:
† Supported by the Science and Technology Commission of Shanghai Municipality, China(No.15JC1490400) and the National Natural Science Foundation of China(No.51373094)

摘要/Abstract

摘要：

在氨基化改性的四氧化三铁(Fe₃O₄)纳米粒子表面引发N-羧酸酐L-谷氨酸苄酯(BLG-NCA)开环聚合, 制得四氧化三铁/聚L-谷氨酸苄酯(PBLG)复合材料(Fe₃O₄-g-PBLG). 改变单体和引发剂比例可以调控Fe₃O₄-g-PBLG的接枝率, 采用热失重分析(TGA)测得实际接枝率分别为66.36%, 79.66%和89.52%. 通过双乳液挥发法制备磁性Fe₃O₄-g-PBLG多孔微载体, 其中接枝率为89.52%的微载体密度为1.034 g/mL, 孔隙率为92.57%, 粒径为200~300 μm, 孔径为40~50 μm, 保水率为370%~400%. 同时Fe₃O₄-g-PBLG微载体具有超顺磁性, 在外加磁场作用下可排布成任意形状, 对治疗复杂结构的骨缺损具有显著优势. 因此Fe₃O₄-g-PBLG多孔微载体在骨组织工程领域具有潜在应用价值.

关键词: 四氧化三铁, 聚L-谷氨酸苄酯, 多孔微载体, 磁性能, 骨组织工程

Abstract:

Ferroferric oxide/poly(γ-benzyl-L-glutamate)(PBLG) composite materials(Fe₃O₄-g-PBLG) was synthesized by initiating γ-benzyl-L-glutamate-N-carboxyanhydride(BLG-NCA) polymerization on the surface of amino-functionalized ferroferric oxide(Fe₃O₄) nanoparticles. Fe₃O₄-g-PBLG composites was confirmed by Fourier transform infrared and X-ray diffraction. Thermogravimetric(TGA) study showed that the grafting ratios of Fe₃O₄-g-PBLG were 66.36%, 79.66%, and 89.52%, and grafting ratio of Fe₃O₄-g-PBLG composite could be controlled. The Fe₃O₄-g-PBLG with grafting ratio of 89.52% was used to fabricate magnetic Fe₃O₄-g-PBLG porous microcarriers. The porous microcarriers possessed suitable density(1.034 g/mL), porosity(92.57%), particle size(200—300 μm), pore size(40—50 μm) and water retention(370%—400%). Meanwhile, microcarriers exhibited superparamagnetism. Under the action of magnetic fields, the microcarriers can be arranged into any shape, showing significant advantages for the treatment of complex structure of the bone defects. These results showed that Fe₃O₄-g-PBLG porous microcarriers might be applied in bone tissue engineering.

Key words: Fe3O4, Poly(-benzyl-L-glutamate)(PBLG), Porous microcarrier, Magnetic property, Bone tissue engineering

中图分类号:

O636

TrendMD:

徐盛华, 夏鹏飞, 张坤玺, 高龙, 尹静波. 四氧化三铁纳米粒子表面接枝聚L-谷氨酸苄酯多孔微载体的构建和性能. 高等学校化学学报, 2017, 38(7): 1295.

XU Shenghua, XIA Pengfei, ZHANG Kunxi, GAO Long, YIN Jingbo. Fabrication and Performance for Fe₃O₄ Nanoparticles Surface Grafted Poly(γ-benzyl-L-glutamate) Porous Microcarriers^†. Chem. J. Chinese Universities, 2017, 38(7): 1295.

图/表 10

Scheme 2 Fabrication of Fe3O4-g-PBLG composites and its porous microcarriers[18]

Fig.1 AFM images of Fe3O4 nano-particles(A), Fe3O4-g-PBLG composite material(B) and 3D structure of Fe3O4-g-PBLG composite material(C)

Fig.2 Diameter of Fe3O4 nano-particles(a) and Fe3O4-g-PBLG composite material(b)

Fig.3 FTIR spectra(A) and XRD patterns(B) of Fe3O4 nano-particles(a), Fe3O4-g-PBLG composite with grafting ratio of 70%(b), 80%(c) and 90%(d) and PBLG(e)

Fig.4 TGA(A) and DSC(B) curves of Fe3O4 nano-particles(a), Fe3O4-g-PBLG composite with grafting ratios of 70%(b), 80%(c) and 90%(d) and PBLG(e)

Fig.5 SEM images of Fe3O4-g-PBLG porous miccarriers with grafting ratios of 70%(A, A'), 80%(B, B') and 90%(C, C')

Fig.6 Porosity and density(A) and water retenticity(B) of Fe3O4-g-PBLG porous microcarriers with different grafting ratios

Fig.7 Hysteresis regression line of Fe3O4 nanoparticles(a) and Fe3O4-g-PBLG porous microcarriers with grafting ratio of 90%(b)

Fig.8 Arrayed behavior of Fe3O4-g-PBLG porous microcarriers with grafting ratio of 90% under different kinds of magnetic field

Fig.9 Confocal laser scanning microscopy images of cell adhesion in Fe3O4-g-PBLG porous microcarriers with grafting ratio of 90%(A) 3D image. Scanning depths in each microcarrier: (B) 1/10 diameter in depth; (C) 2/10 diameter in depth;(D) 3/10 diameter in depth; (E) 4/10 diameter in depth; (F) 5/10 diameter in depth.

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四氧化三铁纳米粒子表面接枝聚L-谷氨酸苄酯多孔微载体的构建和性能

Fabrication and Performance for Fe₃O₄ Nanoparticles Surface Grafted Poly(γ-benzyl-L-glutamate) Porous Microcarriers^†

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四氧化三铁纳米粒子表面接枝聚L-谷氨酸苄酯多孔微载体的构建和性能

Fabrication and Performance for Fe3O4 Nanoparticles Surface Grafted Poly(γ-benzyl-L-glutamate) Porous Microcarriers†

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Fabrication and Performance for Fe₃O₄ Nanoparticles Surface Grafted Poly(γ-benzyl-L-glutamate) Porous Microcarriers^†