HPLC/ESI-Q-TOF MS鉴定淋巴癌患者尿液中的修饰核苷

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (4): 661.doi:

HPLC/ESI-Q-TOF MS鉴定淋巴癌患者尿液中的修饰核苷

李华雨¹, 刘宏民², 卜闪闪¹, 李娟³, 韩冬¹, 吴广银⁴, 王少敏¹, 张明智⁵

1. 郑州大学化学系,
2. 药学院, 郑州 450001;
3. 河北师范大学汇华学院有机化学实验室, 石家庄 050091;
4. 河南省人民医院肿瘤科, 郑州 450003;
5. 郑州大学第一附属医院肿瘤科, 郑州 450001

收稿日期:2008-10-16 出版日期:2009-04-10 发布日期:2009-04-10
通讯作者: 刘宏民, E-mail:liuhm@zzu.edu.cn
基金资助:
国家自然科学基金(批准号: 20272054)资助.

Identified of Urinary Modified Nucleosides of Lymph Cancer Patients by HPLC/ESI-Q-TOF MS

LI Hua-Yu¹, LIU Hong-Min^2*, BU Shan-Shan¹, LI Juan³, HAN Dong¹, WU Guang-Yin⁴, WANG Shao-Min¹, ZHANG Ming-Zhi⁵

1. Department of Chemistry,
2. School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China;
3. Organic Chemistry Laboratory, College of Huihua, Hebei Normal University, Shijiazhuang 050091, China;
4. Oncology Department of the Henan Provincial People′s Hospital, Zhengzhou 450003, China;
5. Oncology Department of the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450001, China

Received:2008-10-16 Online:2009-04-10 Published:2009-04-10
Contact: LIU Hong-Min, E-mail:liuhm@zzu.edu.cn

摘要/Abstract

摘要：

尿液修饰核苷反映了机体RNA的代谢速率及细胞增殖状况, 可以作为非常有发展潜力的肿瘤标志物进行研究. 尿液中的修饰核苷采用Oasis^®HLB固相萃取柱进行纯化, 利用高效液相色谱与电喷雾质谱联用(HPLC-ESI-MS)、高分辨质谱(HRMS)及串联质谱(MS/MS)技术进行分离鉴定. 对淋巴癌患者尿中修饰核苷研究发现, 9种尿液核苷与标样的信息完全一致, 17种无标样的尿液修饰核苷也被鉴定, 其中包括3-甲基腺苷、7-甲基腺嘌呤、5′-脱氢-2′-脱氧次黄苷、3-甲基鸟嘌呤、O6-甲基鸟苷和7-甲基-1-乙基鸟苷6种未见报道的新尿液修饰核苷. 此方法能在无对照品的情况下快速、准确地提纯、分离和鉴定复杂的生物样品.

关键词: HPLC/ESI-Q-TOF MS, 修饰核苷, 淋巴癌病人, 尿液

Abstract:

As modified nucleosides reflect altered tRNA turnover which is impaired in the body of cancer patients, they have been evaluated as potential tumor markers. High performance liquid chromatography(HPLC) was combined with full-scan mass spectrometry, MS/MS analysis and accurate mass measurements in order to identify modified nucleosides which purified from urine. Potential urinary nucleosides were assessed by their evident UV absorbance in the HPLC chromatogram and then further examined by the mass spectrometric techniques. In this manner the information of 9 nucleosides were reproduced with the standard compounds and 17 modified nucleosides without commercial standards were identified in the urine samples from lymph cancer patients including 6 novel urinary modified nucleosides: N3-methyladenosine, N7-methyladenine, 5′-dehydro-2′-deoxyinosine, N3-methylguanine, O6-methylguanosine and N7-methyl-N1-ethylguanosine. This new method is rapid and specific to identify the complex biological samples without control compounds.

Key words: HPLC/ESI-Q-TOF MS, Modified nucleosides, Lymph cancer, Urine

中图分类号:

O657.6

TrendMD:

李华雨, 刘宏民, 卜闪闪, 李娟, 韩冬, 吴广银, 王少敏, 张明智. HPLC/ESI-Q-TOF MS鉴定淋巴癌患者尿液中的修饰核苷. 高等学校化学学报, 2009, 30(4): 661.

LI Hua-Yu, LIU Hong-Min*, BU Shan-Shan, LI Juan, HAN Dong, WU Guang-Yin, WANG Shao-Min, ZHANG Ming-Zhi. Identified of Urinary Modified Nucleosides of Lymph Cancer Patients by HPLC/ESI-Q-TOF MS. Chem. J. Chinese Universities, 2009, 30(4): 661.

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