高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

环三聚磷腈多齿配体的合成及DNA的切割活性

王乐1, 叶勇1, 钟尚宾1, 张迪1, 赵玉芬1,2   

    1. 郑州大学化学系, 河南省化学生物学与有机化学重点实验室, 郑州 450052;
    2. 清华大学化学系, 教育部生命有机磷&化学生物学重点实验室, 北京 100084
  • 收稿日期:2008-06-25 修回日期:1900-01-01 出版日期:2009-03-10 发布日期:2009-03-10
  • 通讯作者: 叶勇, 赵玉芬

Synthesis and DNA-cleaving Function of Cyclotriphosphazene Polydentate Ligands

WANG Le1, YE Yong1*, ZHONG Shang-Bin1, ZHANG Di1, ZHAO Yu-Fen1,2*   

    1. Key Laboratory of Chemical Biology and Organic Chemistry of Henan Province, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China;
    2. Key Laboratory of Bioorganic Phosphorus Chemistry ＆ Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China
  • Received:2008-06-25 Revised:1900-01-01 Online:2009-03-10 Published:2009-03-10
  • Contact: YE Yong, ZHAO Yu-Fen

PDF (PC)

被引次数

10

摘要： 为了得到具有核酸切割功能的人工核酸酶, 设计合成了5种环三聚磷腈多齿配体, 并初步检测了其对DNA的切割活性. 目标化合物的结构由IR, 1H NMR, 31P NMR, 13C NMR和ESI-MS确认. 在生理条件下对pUC19 DNA切割活性的初步实验结果表明, 在化合物5a～5e的Cu(Ⅱ)配合物存在下, 保温24 h后, pUC19 DNA由Form Ⅰ断裂为Form Ⅱ, 即合成目标化合物有明显的DNA切割活性. 同时, 考察了配合物5b+Cu在不同时间下对DNA的切割活性的影响.

关键词: 环三聚磷腈, 多齿配体, pUC19 DNA, 切割

Abstract: A series of multinuclear cyclotriphosphazene ligands were synthesized and used as artificial nuclease enzyme model. All of these new compounds were confirmed by MS, 1H NMR, 31P NMR, 13C NMR and IR. Preliminary studies on the cleavage of pUC19 DNA in the presence of metal complexes were performed. The results strongly suggest that the pUC19 DNA can be cleaved efficiently by these ligands. The effect of DNA cleavage with ligand 5b was also studied under different cleavage time.

Key words: Cyclotriphosphazene, Polydentate ligand, pUC19 DNA, Cleavage

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