高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

正常组织和胰腺癌组织中差异表达蛋白的鉴定

金红1,2, 狄扬3, 陈耀辉2, 傅德良3, 杨芃原1,2   

    1. 复旦大学化学系, 上海 200433;
    2. 复旦大学生物医学研究院, 上海 200032;
    3. 复旦大学附属华山医院, 上海 200030
  • 收稿日期:2008-04-01 修回日期:1900-01-01 出版日期:2009-02-10 发布日期:2009-02-10
  • 通讯作者: 傅德良

Differential Expressed Profile of Proteins in Normal Tissue and Pancreatic Cancer Using Proteomic Approaches

JIN Hong1,2, DI Yang3, CHEN Yao-Hui2, FU De-Liang3*, YANG Peng-Yuan1,2*   

    1. Department of Chemistry, Fudan University, Shanghai 200433, China;
    2. Institute of Biomedical Science, Fudan University, Shanghai 200032, China;
    3. Affiliated Huashan Hospital, Fudan University, Shanghai 200030, China
  • Received:2008-04-01 Revised:1900-01-01 Online:2009-02-10 Published:2009-02-10
  • Contact: FU De-Liang

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被引次数

12

摘要： 采用双向凝胶电泳和生物质谱技术, 对12对胰腺癌组织和癌旁组织样品、3个胰腺良性疾病样品、3个正常胰腺组织样品的蛋白质进行了分离和鉴定, 获得了重复性较好的双向凝胶电泳图谱; 鉴定了胰腺癌和癌旁组织的差异表达蛋白质, 发现了30个差异表达蛋白质; 应用MALDI-TOF-MS/MS技术对差异表达蛋白质进行鉴定, 共有24个蛋白质得到鉴定, 其中15个蛋白质在胰腺癌组织中表达上调, 9个蛋白质表达下调. 这些蛋白质与胰腺癌的发生相关, 可能成为胰腺癌的分子标志物和药物治疗的靶蛋白.

关键词: 胰腺癌, 蛋白质组差异表达谱, 双向凝胶电泳, 生物质谱

Abstract: The pancreatic cancer is one of highly malignant tumor with high morbidity rate in recent years disease incidence rate constant rise. Due its difficulty of early diagnosis, and the deep hideaway position, the cure rate and 5 years survival rate for this disease is very low. The pathogenesis of pancreatic cancer is still unknown. In this study pancreatic cancer and normal tissue(tissue nearby cancer) were used to run differential protein expression profile via 2DE and MALDI-TOF-MS-MS. Among 30 differential expressed proteins, 24 proteins are identified. 15 proteins in pancreatic cancer are up-regulated, 9 proteins are down-regulated. These proteins are probably correlated with the causes of disease and can eventually become disease biomarker and treatment target protein.

Key words: Pancreatic cancer, Differential protein expression profile, Two-dimensional gel electrophoresis, Bio-MS

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