分子印迹复合膜对降糖药的拉曼光谱快速检测

doi:10.7503/cjcu20150533

高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (2): 239.doi: 10.7503/cjcu20150533

分子印迹复合膜对降糖药的拉曼光谱快速检测

贾华¹, 尹瑞林^1,,A;^*, 钟旭², 薛敏¹(), 赵瑜³, 孟子晖¹(), 汪群杰²

1. 北京理工大学化工与环境学院, 北京 100081
2. 天津博纳艾杰尔科技有限公司, 天津 300462
3. 中国食品药品检定研究院, 北京 100050

收稿日期:2015-07-09 出版日期:2016-02-10 发布日期:2015-12-26
通讯作者: 尹瑞林 E-mail:minxue@bit.edu.cn;m_zihui@yahoo.com
作者简介:联系人简介: 薛敏, 女, 博士, 讲师, 主要从事样品前处理和色谱分析方面的研究. E-mail:minxue@bit.edu.cn;孟子晖, 男, 博士, 教授, 博士生导师, 主要从事光子晶体方面的研究. E-mail:m_zihui@yahoo.com;* 现在北京101中学.
基金资助:
国家自然科学基金(批准号: 21375009)资助

Anti-diabetic Drugs Detection by Raman Spectrometry with Molecular Imprinted Composite Membrane^†

JIA Hua¹, YIN Ruilin¹, ZHONG Xu², XUE Min^1,^*(), ZHAO Yu³, MENG Zihui^1,^*(), WANG Qunjie²

1. School of Chemical & Environmental Engineering, Beijing Institute of Technology, Beijing 100081, China
2. Bonna-Agela Technologies, Tianjin 300462, China
3. National Institutes for Food and Drug Control, Beijing 100050, China

Received:2015-07-09 Online:2016-02-10 Published:2015-12-26
Contact: XUE Min,MENG Zihui E-mail:minxue@bit.edu.cn;m_zihui@yahoo.com
Supported by:
† Supported by the National Natural Science Foundation of China(No.21375009)

摘要/Abstract

摘要：

基于分子印迹技术的特异性识别特点, 以假模板盐酸胍和4-(2-氨乙基)苯磺酰胺制备了2种分子印迹复合膜, 用其对保健品中的二甲双胍、苯乙双胍和格列本脲进行吸附后, 采用拉曼光谱法实现了保健品中降糖药的富集与快速检测. 考察了膜制备过程中纳米银的加入对拉曼光谱的增强作用, 以及吸附样品浓度等的影响. 实验结果表明, 盐酸胍分子印迹复合膜吸附5 mg/mL以上的二甲双胍或苯乙双胍后具有明显的拉曼响应, 4-(2-氨乙基)苯磺酰胺印迹膜吸附10 mg/mL格列本脲后具有明显的拉曼响应. 所建立的分子印迹复合膜结合拉曼光谱检测方法可用于多种实际样品的检测.

关键词: 保健品, 降糖药, 分子印迹复合膜, 纳米银, 拉曼光谱

Abstract:

Some artificially synthesized anti-diabetic drugs are frequently found in health products to enhance their curative efficacy. Based on the specific recognition characteristics of molecularly imprinted technology, two kinds of molecularly imprinted composite membranes were prepared with dummy template of guanidine hydrochloride and 4-(2-aminoethyl)-benzene sulfonamide. The adsorption capacities of these two membranes to metformin hydrochloride, phenformin hydrochloride and glibenclamide were studied. Meanwhile, surface enhancement Raman spectrometry(SERS) to determine the target chemicals was established and employed in the membrane detection after infiltrated in the health products extract solution. The influence of nano-silver particles on the membrane to SERS was also studied. The detection limits of metformin hydrochloride, phenformin hydrochloride and glibenclamide are 5, 5 and 10 mg/mL, respectively. This method was also proved by several pieces of real samples.

Key words: Health care product, Anti-diabetic drug, Molecularly imprinted membrane, Nano-silver, Raman spectroscopy

中图分类号:

O657.37

TrendMD:

贾华, 尹瑞林, 钟旭, 薛敏, 赵瑜, 孟子晖, 汪群杰. 分子印迹复合膜对降糖药的拉曼光谱快速检测. 高等学校化学学报, 2016, 37(2): 239.

JIA Hua, YIN Ruilin, ZHONG Xu, XUE Min, ZHAO Yu, MENG Zihui, WANG Qunjie. Anti-diabetic Drugs Detection by Raman Spectrometry with Molecular Imprinted Composite Membrane^†. Chem. J. Chinese Universities, 2016, 37(2): 239.

图/表 11

Fig.1 Chemical structures of dummy templates and anti-diabetic drugs

Fig.2 UV-Vis spectrum of nano-silver particles

Fig.3 SEM image of nano-silver particles

Fig.4 SEM images of membrane matrix(A), MIM(B) and MIM-Ag(C)

Fig.5 Raman spectra of metformin hydrochloride(A), MIM(a, c) and MIM-Ag(b, d) before(a, b) and after(c, d) adsorption of 75 mg/mL metformin hydrochloride(B)

Fig.6 Raman spectrum of phenformin hydrochloride

Fig.7 Raman spectra of MIM-Ag before and after adsorption of different concentrations of metformin hydrochloride(A) and phenformin hydrochloride(B) a. MIM-Ag(before adsorption); concentration/(mg·mL-1), b—g: 5, 9, 19, 38, 75, 100.

Fig.8 Raman spectra of MIM-Ag before(a) and after(b) adsorption of a real sample (No.JC1012998) extract

Fig.9 Raman spectra of MIM-Ag after adsorption of a real sample(No.JC1012998) extract(a) and rinsed with chloroform(b)

Fig.10 Raman spectra of glibenclamide(A) and B-MIM-Ag before(a) and after adsorption(b—d)(B) (B) b—d. B-MIM-Ag adsorbs 5, 12 and 15 mg/mL gibenciamide, respectively.

Fig.11 Raman spectra of B-MIM-Ag before(a) and after adsorption of a real sample (No.JC1300635) extract(b) and after adsorption of the sample(No.JC1300635) extract added standard(c)

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分子印迹复合膜对降糖药的拉曼光谱快速检测

Anti-diabetic Drugs Detection by Raman Spectrometry with Molecular Imprinted Composite Membrane^†

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分子印迹复合膜对降糖药的拉曼光谱快速检测

Anti-diabetic Drugs Detection by Raman Spectrometry with Molecular Imprinted Composite Membrane†

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Anti-diabetic Drugs Detection by Raman Spectrometry with Molecular Imprinted Composite Membrane^†