高等学校化学学报

高等学校化学学报

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基于超氧化物歧化酶/氧化锌的超氧离子传感器

邓子峰, 徐伟, 杨晓   

  1. 同济大学化学系, 上海 200092
  • 收稿日期:2008-07-27 修回日期:1900-01-01 出版日期:2009-01-10 发布日期:2009-01-10
  • 通讯作者: 杨晓

Superoxide Biosensor Based on Superoxide Dismutase/Nano-ZnO

DENG Zi-Feng, XU Wei, YANG Xiao*   

  1. Department of Chemistry, Tongji University, Shanghai 200092, China
  • Received:2008-07-27 Revised:1900-01-01 Online:2009-01-10 Published:2009-01-10
  • Contact: YANG Xiao

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被引次数

4

摘要： 利用物理气相沉积法制备了纳米氧化锌(Nano-ZnO) 膜, 通过扫描电子显微镜(SEM)、紫外-可见分光光度法(UV-Vis)、X 射线衍射(XRD)及电化学等方法测定了其物理化学性质. 实验结果表明, 该Nano-ZnO 膜是具有多晶六边形纤维锌矿结构的多孔纳米膜, 微粒直径在 50～100 nm, 室温禁带宽度3.37 eV. 采用浸渍法将超氧化物歧化酶(SOD)直接修饰于 Nano-ZnO 膜上, 制备了SOD 修饰电极(SOD/ZnO). 通过交流阻抗法(EIS)及循环伏安法(CV)证明了SOD能稳定地吸附在多孔ZnO膜上, 并实现了直接电子传递; 紫外-可见及红外光谱研究证明吸附在ZnO膜上的蛋白质保持了良好的生物催化活性, 并成功地构建了第三代超氧离子(O2-)生物传感器. 这种生物传感器有较宽的线性范围(氧化电流: 0.24~180×10-6 mol/L, 还原电流: 0.12~250×10-6 mol/L)、较低的检测限(氧化电流: 2×10-7 mol/L, 还原电流: 1×10-7 mol/L)、较快的响应时间(4 s)以及较好的稳定性.

关键词: 氧化锌, 超氧化物歧化酶, 超氧离子, 生物传感器

Abstract: A nanoporous ZnO film(nano-ZnO) was prepared by physical vapor deposited method and its physical and chemical properties were studied by means of SEM, UV-Vis, XRD and electrochemical methods. The results show that nano-ZnO is a nanoporous film with a polycrystalline hexagonal wurtzite structure. The average diameter of the nanoparticles is about 50—100 nm and its band gap is 3.37 eV. A new superoxide dismutase(SOD) modified nano-ZnO electrode(SOD/ZnO) was prepared by dipping method. SOD was modified stably in the nano-ZnO film confirmed by electrochemical impedance spectroscopy(EIS) and the direct electron transfer could be achieved confirmed by cyclic voltammetry(CV). Spectrum studies indicated that SOD kept its bio-activities. A novel third-generation O2- biosensor with high selectivity, quick response time(4 s), large linear range(anodic current response: 0.24—180×10-6 mol/L; cathodic current response: 0.12—250×10-6 mol/L) and good sensitivity(anodic: 2×10-7 mol/L; cathodic: 1×10-7 mol/L) was constructed and its analytical properties were studied.

Key words: ZnO, Superoxide dismutase, Superoxide anion, Biosensor

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