高等学校化学学报

高等学校化学学报

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海洋放线菌Streptomyces sp. 124092中的细胞毒活性成分

解修超1,2, 梅文莉1, 曾艳波1, 林海鹏1, 庄令1, 戴好富1, 洪葵1   

    1. 中国热带农业科学院热带生物技术研究所, 海口 571101;
    2. 陕西理工学院陕西省资源生物重点实验室, 汉中 723001
  • 收稿日期:2008-03-03 修回日期:1900-01-01 出版日期:2008-11-10 发布日期:2008-11-10
  • 通讯作者: 戴好富, 洪葵

Cytotoxic Constituents from Marine Actinomycete Streptomyces sp. 124092

XIE Xiu-Chao1,2, MEI Wen-Li1, ZENG Yan-Bo1, LIN Hai-Peng1, ZHUANG Ling1, DAI Hao-Fu1*, HONG Kui1*   

    1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;
    2. Shanxi Key Laboratory of Resource Biology, Shanxi University of Technology, Hanzhong 723001, China
  • Received:2008-03-03 Revised:1900-01-01 Online:2008-11-10 Published:2008-11-10
  • Contact: DAI Hao-Fu, HONG Kui

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摘要： 对海洋放线菌Streptomyces sp.发酵液的提取物进行柱层析分离, 得到5个化合物. 经MS, NMR, 1H-1H COSY, HSQC和HMBC等数据鉴定, 其结构分别为3-氨基-丙酸对甲苯酯(1)、6-Amino-3-(4-hydroxybenzyl)-1,4-diazonane-2,5-dione(2)、正丁基-α-D-吡喃甘露糖苷(3)、大豆苷元(4)和1H-3-吲哚甲酸(5). 其中化合物1和2为新化合物, 细胞毒活性测试结果表明, 化合物1~4对人肝癌细胞(SMMC-7721)具有不同程度的生长抑制活性.

关键词: 海洋放线菌, Streptomyces sp., 细胞毒活性, 分离和纯化

Abstract: To study the cytotoxic constituents from marine actinomycete Streptomyces sp.(124092) of rhizosphere soil of mangrove plant Heritiera littoralis, five compounds were isolated by column chromatography from the fermentation broth of marine actinomycete Streptomyces sp.(124092). Their structures were elucidated as p-tolyl 3-aminopropanoate(1), 6-amino-3-(4-hydroxybenzyl)-1,4-diazonane-2,5-dione(2), n-butyl mannoside(3), daidzein(4), and indole-β-carboxylic acid(5), by MS, NMR, 1H-1H COSY, HMQC, and HMBC spectra. Among them, compounds 1 and 2 are new compounds. Cytotoxic bioassay results show that compounds 1—4 possess inhibitory effects on human hepatoma SMMC-7721 cell line in vitro.

Key words: Marine actinomycete, Streptomyces sp., Cytotoxicity, Isolation and purification

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