高等学校化学学报

高等学校化学学报 ›› 1993, Vol. 14 ›› Issue (9): 1320.

• 研究论文 • 上一篇    下一篇

黑木耳酸性杂多糖溶液性质研究

张俐娜, 陈和生   

  1. 武汉大学化学系, 武汉 430072
  • 收稿日期:1992-11-28 修回日期:1993-05-09 出版日期:1993-09-24 发布日期:1993-09-24
  • 通讯作者: 张俐哪.
  • 作者简介:第一作者:女, 53岁, 教授.
  • 基金资助:

    国家自然科学基金

Studies on Solution Properties of Acidic Heteropolysaccharide of Auricularia Auricula-Judae

ZHANG Li-Na, CHEN He-Sheng   

  1. Department of Chemistry, Wuhan University, Wuchang, 430072
  • Received:1992-11-28 Revised:1993-05-09 Online:1993-09-24 Published:1993-09-24

PDF (PC)

被引次数

60

摘要： 从黑木耳子实体提取出的酸性杂多糖,通过重沉淀分成8个级分,用小角激光光散射和膜渗透压法测得试样在0.5mol/LNaCl/10％镉乙二胺饱和液(C)中的Mw(5.88×105)、Mn(1.43×105)和A2值.研究了各个分级试样在3种水溶液中30℃的粘度行为,确立了Mark-Houwink方程为:(A)0.5mol/LNaCl,[η]=0.235Mw0.48;(B)0.2molNa2SO4/0.01mol/L磷酸盐缓冲液,[η]=7.61×10-2Mw0.57;(C)[η]=6.44×10-2Mw0.59.无扰尺寸(S2)0/M及K8为12.9×10-18cm2·mol/g及0.175cm3/g.确证该多糖分子在溶液中呈稍刚硬的柔性无规线团状态.

关键词: 黑木耳多糖, 光散射法, 膜渗透压法, 无扰尺寸, 溶液性质

Abstract: Aacidic heteropolysaccharide (F Ⅱ), which is composed of D-mannose, D-xylose, D-glucose and glucuronic acid, was isolated from the fruiting body of Auricularia auricula-judae, and separated into 8 fractions by reprecipitation.The weight average molecular weights Mw, number average molecular weights Mn and second viriai coefficients A2 of fractions and unfrac-tioned samples in 0.5 mol/L NaCl/10%cadoxen (C) were measured by low angle laser light scattering and membrane osmometry.The values of Mw and Mn of the acidic heteropolysaccharide were obtained to be:5.88×105 and 1.43 ×105.Intrinsic viscosities [η] were investigated on fractioned samples in aqueous 0.5 mol/L NaCl (A), 0.2 mol/L Na2SO4/0.01 mol/Lphosphate buffer (B) and (C) at 30℃.The Mark-Houwink equations were established as [η] = 0.235 Mw0.48 (A), [η] = 7.61×10-2Mw0.57 (B) and [η] = 6.44×10-2Mw0.59 (C).The unperturbed dimensions〈S20 and Kθ are respectively 12.1 ×10-18 cm2mol g-1 and 0.175 cm3g-1.We conclude that the acidic heteropolysaccharide behaves as a slightly stiffened random coil chain in aqueous solution.

Key words: Auricularia auricula-judae polysaccharide, Light scattering, Membrane osmometry, Unperturbed dimension, Solution property

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