紫外光辐射山茱萸水提液制备纳米银及生物活性

doi:10.7503/cjcu20190721

高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (6): 1391.doi: 10.7503/cjcu20190721

紫外光辐射山茱萸水提液制备纳米银及生物活性

魏思敏¹,王英辉^2,^*(),唐志书^1,^*(),苏瑞¹,胡锦航¹,郭惠¹,李琛¹,蒋金涛¹,宋忠兴¹

^1. 陕西中医药大学, 陕西省中药资源产业化协同创新中心, 秦药特色资源研究开发国家重点实验室(培育), 陕西省创新药物研究中心, 咸阳 712083
^2. 长安大学理学院, 西安 710064

收稿日期:2019-12-31 出版日期:2020-06-10 发布日期:2020-03-06
通讯作者: 王英辉,唐志书 E-mail:wangyinghui@iccas.ac.cn;tzs6565@163.com
基金资助:
国家自然科学基金(21705029);国家自然科学基金(21701131);陕西省高校科协青年人才托举计划(20190307);长安大学中央高校基本科研业务费专项资金(300102120303)

Novel Biosynthesis Method of Silver Nanoparticle by UV Radiation of Cornus Officinalis Aqueous Extract and Biological Activities ^†

WEI Simin¹,WANG Yinghui^2,^*(),TANG Zhishu^1,^*(),SU Rui¹,HU Jinhang¹,GUO Hui¹,LI Chen¹,JIANG Jintao¹,SONG Zhongxing¹

^1. Shaanxi Collaborative Innovation Center of Chinese Medicine Resources Industrialization, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources(Cultivation), Shaanxi Innovative Drug Research Center, Shaanxi University of Chinese Medicine, Xianyang 712083, China
^2. College of Science, Chang’an University, Xi’an 710064, China

Received:2019-12-31 Online:2020-06-10 Published:2020-03-06
Contact: Yinghui WANG,Zhishu TANG E-mail:wangyinghui@iccas.ac.cn;tzs6565@163.com
Supported by:
† National Natural Science Foundation of China(21705029);National Natural Science Foundation of China(21701131);Young Talent Fund of University Association for Science and Technology in Shaanxi Province, China(20190307);Fundamental Research Funds for the Central Universities, Chang’an University, China(300102120303)

摘要/Abstract

摘要：

利用254 nm紫外光照射山茱萸水提液在室温下制备了纳米银, 并通过UV-Vis光谱检测其在410 nm附近的等离子体共振峰; 研究了溶液pH值、料液比以及反应时间对还原反应的影响, 确定了纳米银的最优合成条件: pH=7.0, 料液比1∶1, 反应时间1 h. 通过X射线晶体衍射、透射电子显微镜和激光粒度仪对纳米银的晶体结构、粒径、表面性质和形貌等进行表征发现, 在最优反应条件下制得的纳米银为面心立方结构, 呈近球形, 平均粒径(55.4±0.9) nm, 分散均匀, 表面带负电(-10.2 mV), 具有较高的稳定性. 生物活性研究结果表明, 制得的纳米银具有良好的抗氧化、抗菌及抗癌活性. 当纳米银浓度为62.5 μg/mL时, 对DPPH自由基的清除率为70.0%; 对S. aureus和E. coli最低抑菌浓度分别为3.9和7.8 μg/mL; 对结直肠癌细胞HCT116和SW620的IC₅₀值分别为23.1和35.1 μg/mL.

关键词: 山茱萸, 紫外光照射法, 纳米银, 生物活性

Abstract:

Owing to the widely applications of silver nanoparticles(AgNPs) in catalysis and biomedicine, researchers have focused their attentions on the formation method. In this work, a green and easy synthesis method of silver nanoparticles(AgNPs) by 254 nm ultraviolet lamp radiation using Cornus officinalis aqueous extract was reported, and the antioxidant, antibacterial and anticancer activities were assessed. The influence of the solution pH(7.0), material proportion(1∶1) and reaction time(1 h) on reduction reaction were discussed to confirm the most efficient reaction condition. X-ray diffraction(XRD), dynamic light scattering and transmission electron microscopy were conducted to investigate the shape, size and surface of the silver nanoparticles, revealing a face-centered cubic(fcc) structure and spherical shape with an average particle size of (55.4±0.9) nm covered by anion(Zeta potential: -10.2 mV). By calculating the free radical scavenging rate for DPPH, the antioxidant activities of AgNPs were assessing. When the concentration of AgNPs increased to 62.5 μg/mL, the free radical scavenging rate was 70% meaning a good antioxidant activity. Furthermore, the AgNPs generated by Cornus officinalis aqueous extract revealed significant inhibition on the growth of S. aureus and E. coli, where the MIC values were 3.9 and 7.8 μg/mL, respectively. The IC₅₀ values for colorectal cancer cell HCT116 and SW620 were 23.1 and 35.1 μg/mL, respectively, indicating a good anticancer activity.

Key words: Cornus officinalis, Ultrasonic radiation, Silver nanoparticles, Biological activity

中图分类号:

O614
O644

TrendMD:

魏思敏,王英辉,唐志书,苏瑞,胡锦航,郭惠,李琛,蒋金涛,宋忠兴. 紫外光辐射山茱萸水提液制备纳米银及生物活性. 高等学校化学学报, 2020, 41(6): 1391.

WEI Simin,WANG Yinghui,TANG Zhishu,SU Rui,HU Jinhang,GUO Hui,LI Chen,JIANG Jintao,SONG Zhongxing. Novel Biosynthesis Method of Silver Nanoparticle by UV Radiation of Cornus Officinalis Aqueous Extract and Biological Activities ^†. Chem. J. Chinese Universities, 2020, 41(6): 1391.

图/表 8

Fig.1 UV-Vis spectra of the mixtures after the reaction between Cornus officinalis aqueous extract and AgNO3 at different pH values(A) and the size of as-synthesized AgNPs(B)

Fig.2 UV-Vis spectra of the mixtures after the reaction between Cornus officinalis aqueous extract and AgNO3 with different volume ratios

Fig.3 UV-Vis spectra of the mixtures after the reaction between Cornus officinalis aqueous extract and AgNO3 at different times

Fig.4 XRD pattern(A) and TEM image(B) of the synthesized AgNPs

Fig.5 Size and zeta potential of synthesized AgNPs

Fig.6 Antibacterial activity for S. aureus(A) and E. coli(B) of AgNPs generating by Cornus officinalis aqueous extract

Fig.7 Free radical scavenging rate of AgNPs against DPPH

Fig.8 Inhibition effects of AgNPs on HCT116(A) and SW620(B)

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紫外光辐射山茱萸水提液制备纳米银及生物活性

Novel Biosynthesis Method of Silver Nanoparticle by UV Radiation of Cornus Officinalis Aqueous Extract and Biological Activities ^†

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紫外光辐射山茱萸水提液制备纳米银及生物活性

Novel Biosynthesis Method of Silver Nanoparticle by UV Radiation of Cornus Officinalis Aqueous Extract and Biological Activities †

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Novel Biosynthesis Method of Silver Nanoparticle by UV Radiation of Cornus Officinalis Aqueous Extract and Biological Activities ^†