离子液体[MBPy]Tf2N的光谱特征及荧光法检测

doi:10.7503/cjcu20150242

高等学校化学学报 ›› 2015, Vol. 36 ›› Issue (10): 1906.doi: 10.7503/cjcu20150242

离子液体[MBPy]Tf₂N的光谱特征及荧光法检测

欧阳巧凤^1,², 郑兰兰^1,⁴, 曹红²(), 张馨¹, 李春³, 沈姣², 陈礼婷², 刘进²

1. 石河子大学化学化工学院, 新疆兵团化工绿色重点实验室-省部共建国家重点实验室培育基地,石河子 832003
2. 嘉兴学院生物与化学工程学院, 嘉兴 314001
3. 北京理工大学生命学院, 北京 100081
4. 喀什大学, 喀什 844006

收稿日期:2015-03-26 出版日期:2015-10-10 发布日期:2015-09-14
作者简介:联系人简介: 曹红, 女, 博士, 副教授, 主要从事生物催化与酶工程的研究. E-mail:chyf_ch@126.com
基金资助:
国家自然科学基金(批准号: 21146012, 21266029)、教育部科学技术研究重点项目(批准号: 209146)和新疆生产建设兵团博士资金项目(批准号: 2009JC13)资助

Spectral Performance and Fluorescence Detection of Ionic Liquid [MBPy]Tf₂N^†

OUYANG Qiaofeng^1,², ZHENG Lanlan^1,⁴, CAO Hong^2,^*(), ZHANG Xin¹, LI Chun³, SHEN Jiao², CHEN Liting², LIU Jin²

1. School of Chemistry and Chemical Engineering, Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi 832003, China
2. College of Biological and Chemical Engineering, Jiaxing University, Jiaxing 314001, China
3. School of Life Science, Beijing Institute of Technology, Beijing 100081, China
4. Kashgar University, Kashi 844006, China

Received:2015-03-26 Online:2015-10-10 Published:2015-09-14
Contact: CAO Hong E-mail:chyf_ch@126.com
Supported by:
† Supported by the National Natural Science Foundation of China(Nos.21146012, 21266029), the Science and Technology Foundation of Ministry of Education, China(No.209146) and the Doctoral Foundation Project of Xinjiang Production and Construction Corps of China(No.2009JC13)

摘要/Abstract

摘要：

为满足疏水性离子液体N-甲基-N-丁基吡咯烷双三氟甲烷磺酰亚胺盐([MBPy]Tf₂N)在甘草酸生物催化体系中分析检测的需要, 研究了[MBPy]Tf₂N的光谱特征, 并考察了溶剂、醇-水混合溶剂和pH值对[MBPy]Tf₂N荧光特性的影响, 建立了生物催化体系中[MBPy]Tf₂N的荧光光谱检测方法. 研究结果表明, [MBPy]Tf₂N在186 nm处有1个最大吸收峰, 常规紫外检测法不适于对其进行检测; [MBPy]Tf₂N荧光光谱最大激发波长为228 nm, 发射波长为340 nm, 且其耐光褪色性良好, 在水溶液中荧光量子产率为0.067, 在醇-水混合溶剂中其荧光强度有增大趋势, 在碱性介质中其荧光强度受影响较大; 荧光光谱法检测[MBPy]Tf₂N的检出限(S/N=3)为1.00 μg/mL, 标准曲线在5.00~1.25×10³ μg/mL浓度范围内线性关系良好(R²=0.9999), 生物反应液中加标回收率为97.4%~107.4%(相对标准偏差RSD=4.6%).

关键词: 疏水性离子液体, N-甲基-N-丁基吡咯烷双三氟甲烷磺酰亚胺盐([MBPy]Tf2N), 光谱特征, 荧光检测法, 生物催化体系

Abstract:

The detection method of hydrophobic ionic liquid N-methyl-N-butyl pyrrolidinium bis[(trifluoro-methyl)sulfonyl]imide([MBPy]Tf₂N) was established by means of fluorescence spectrum and applied for the analysis of [MBPy]Tf₂N from glycyrrhizin biological catalysis system containing ionic liquid. The effects of solvent, ethanol-water mixture solvent and pH on fluorescence spectrum were explored. Spectral features of [MBPy]Tf₂N, the maximum absorption wavelength at 186 nm, were apparently cannot detect [MBPy]Tf₂N by common UV method. The maximum excitation wavelength of [MBPy]Tf₂N is at 228 nm and the maximum emission wavelength of it is at 340 nm, which can well againsted photobleaching and the fluorescence quantum yield was 0.067 in water. The fluorescence intensity increased in ethanol-water mixture solvent and changed much in alkaline medium. The fluorescence detection method for [MBPy]Tf₂N had a wide linear range over [MBPy]Tf₂N concentration from 5.00 to 1.25×10³μg/mL with a detection limit of 1.00 μg/mL(S/N=3). The spiked recoveries were from 97.4% to 107.4%(RSD=4.6%) in glycyrrhizin biological catalysis system.

Key words: Hydrophobic ionic liquid, N-Methyl-N-butyl pyrrolidinium bis[(trifluoromethyl)sulfonyl]imide([MBPy]Tf2N), Spectral characterization, Fluorescence detection method, Biological catalysis system

中图分类号:

O657.3

TrendMD:

欧阳巧凤, 郑兰兰, 曹红, 张馨, 李春, 沈姣, 陈礼婷, 刘进. 离子液体[MBPy]Tf₂N的光谱特征及荧光法检测. 高等学校化学学报, 2015, 36(10): 1906.

OUYANG Qiaofeng, ZHENG Lanlan, CAO Hong, ZHANG Xin, LI Chun, SHEN Jiao, CHEN Liting, LIU Jin. Spectral Performance and Fluorescence Detection of Ionic Liquid [MBPy]Tf₂N^†. Chem. J. Chinese Universities, 2015, 36(10): 1906.

图/表 7

Fig.1 UV absorption spectra of ionic liquid [MBPy]Tf2N in acetonitrilec/(μg·mL-1): a—g. 0, 50.05, 100.10, 150.15,

Fig.2 Emission spectra of [MBPy]Tf2N in ethanol(a), water(b) and dichloromethane(c)

Fig.3 Emission spectra of [MBPy]Tf2N and relative linearities(insets) in ethanol(A), water(B) and dichloromethane(C)

Fig.4 Photobleaching properties of [MBPy]Tf2N in water(A) Time-based fluorescence steady-state measurements of [MBPy]Tf2N in water; (B) emission spectra of [MBPy]Tf2N before(a) and after(b) photobleaching in water.

Fig.5 Effect of ethanol content on [MBPy]Tf2N fluorescenceInset is the [MBPy]Tf2N fluorescence vs. ethanol content of 80%—100%.

Fig.6 Effect of pH on [MBPy]Tf2N fluorescence

Table 1 Recoveries of [MBPy]Tf2N in bioreaction liquid

Sample	Original/(μg·mL^-1)	Spiked/(μg·mL^-1)	Found/(μg·mL^-1)	Recovery(%)	RSD(%)(n=3)
1	126.05	50.02	179.77	107.4	4.6
2	126.05	100.04	223.49	98.81	3.6
3	163.09	150.06	314.05	100.6	2.2

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离子液体[MBPy]Tf₂N的光谱特征及荧光法检测

Spectral Performance and Fluorescence Detection of Ionic Liquid [MBPy]Tf₂N^†

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