汞离子对细胞代谢通路影响的代谢组学

高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (6): 1146.

汞离子对细胞代谢通路影响的代谢组学

张瑞兴^1,2, 刘舒¹, 皮子凤¹, 宋凤瑞¹, 刘志强¹

1. 中国科学院长春应用化学研究所, 长春质谱中心&吉林省中药化学与质谱重点实验室, 长春 130022;
2. 中国科学院大学, 北京 100049

收稿日期:2014-03-03 出版日期:2014-06-10 发布日期:2014-05-12
作者简介:宋凤瑞，女，博士，研究员，博士生导师，主要从事天然产物质谱、代谢及代谢组学研究.E-mail：songfr@ciac.ac.cn
基金资助:
国家自然科学基金（批准号：81373952）资助.

Cell Metabolomics Study of Hg(Ⅱ) Ion Effect on the Metabolic Pathways of Cells

ZHANG Ruixing^1,2, LIU Shu¹, PI Zifeng¹, SONG Fengrui¹, LIU Zhiqiang¹

1. National Center of Mass Spectrometry in Changchun & Jilin Province Key Laboratory of Chinese Medicine and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. University of the Chinese Academy of Sciences, Beijing 100049, China

Received:2014-03-03 Online:2014-06-10 Published:2014-05-12
Supported by:
Supported by the National Natural Science Foundation of China(No.81373952).

摘要/Abstract

摘要：

利用超高效液相色谱-四级杆飞行时间质谱（UPLC-Q-TOF-MS）技术对汞离子作用后细胞的代谢组学进行了研究，结合化学计量学方法，对代谢组学数据进行了多维统计分析.结果表明，与非汞离子作用组相比，汞离子作用组存在能量、磷脂、脂肪酸及氨基酸代谢异常，并发现16种有显著差异的生物标记物.进一步探讨了汞中毒的细胞代谢机理及细胞的应激保护.本研究建立的基于UPLC-Q-TOF-MS技术的细胞代谢组学快速分析方法可以对细胞在重金属作用后的代谢物变化进行轮廓分析.

关键词: 细胞代谢组学, 超高效液相色谱-四级杆飞行时间质谱, 汞毒性, 代谢应激

Abstract:

Mercury is harmful for human that can cause many diseases. But how it causes the disease and if any protective response from cell metabolism happens remain unclear till now. In this work, we analyzed the metabolomics of Hela cells treated with Hg(Ⅱ) ion by ultra-performance liquid chromatography-high resolution mass spectrometry. Totally 16 metabolites have been found to change significantly during Hg(Ⅱ) ion treatment by the facility of orthogonal partial least squares discriminant analysis(OPLS-DA). The results showed that Hg(Ⅱ) ion could impact many metabolic reactions including energy metabolism, phospholipid metabolism, fatty acid metabolism, amino acid metabolism and nucleic acid metabolism. But cells also had some stress reactions to protect themselves from Hg(Ⅱ) ion damage.

Key words: Cell metabolomics, Ultra-performance liquid chromatography quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS), Mercury toxicity, Metabolic stress

中图分类号:

O657

TrendMD:

张瑞兴, 刘舒, 皮子凤, 宋凤瑞, 刘志强. 汞离子对细胞代谢通路影响的代谢组学. 高等学校化学学报, 2014, 35(6): 1146.

ZHANG Ruixing, LIU Shu, PI Zifeng, SONG Fengrui, LIU Zhiqiang. Cell Metabolomics Study of Hg(Ⅱ) Ion Effect on the Metabolic Pathways of Cells. Chem. J. Chinese Universities, 2014, 35(6): 1146.

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