高等学校化学学报

高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (1): 50.doi: 10.7503/cjcu20120185

• 研究论文: 无机化学 • 上一篇    下一篇

双亲性氧化石墨烯的合成及生物相容性

李宁1,2,3, 张琦2, 张庆庆2,3, 宋琴2, 齐琳2, 王龙2,3, 唐明亮2, 靳刚1,3, 程国胜2,3   

  1. 1. 中国科学院力学研究所, 北京 100190;
    2. 中国科学院苏州纳米技术与纳米仿生研究所纳米生物医学部, 苏州 215123;
    3. 中国科学院大学, 北京 100049
  • 收稿日期:2012-03-05 发布日期:2012-12-31
  • 通讯作者: 程国胜,男,博士,研究员,主要从事纳米-干细胞界面研究.E-mail:gscheng2006@sinano.ac.cn E-mail:gscheng2006@sinano.ac.cn
  • 基金资助:

    国家自然科学基金青年科学基金(批准号: 31101059)资助.

Synthesis and Biocompatibility of Amphiphilic Graphene Oxide

LI Ning1,2,3, ZHANG Qi2, ZHANG Qing-Qing2,3, SONG Qin2, QI Lin2, WANG Long2,3, TANG Ming-Liang2, JIN Gang1,3, CHENG Guo-Sheng2,3   

  1. 1. Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China;
    2. Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China;
    3. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2012-03-05 Published:2012-12-31

PDF (PC)

被引次数

18

摘要：

以六亚甲基二异氰酸酯(HMDI)为偶联剂, 通过与氧化石墨烯(GO)中的羧基或羟基反应, 形成酰胺键或氨基甲酸酯键活化GO, 然后与TWEEN 80中的羟基反应, 将双亲性TWEEN分子偶联于GO表面, 制备了双亲性GO. 所制备的TWEEN 80接枝GO在水、 氯仿和己烷等溶剂中均可稳定分散. 以PC12及HeLa细胞为细胞模型, 考察TWEEN 80接枝GO所制薄膜的细胞毒性, 形态学观察及细胞活性检测(Calcein AM & EthD-1荧光染色及MTT检测)结果表明其具有良好的生物相容性.

关键词: 双亲性氧化石墨烯, 生物相容性, TWEEN 80, 六亚甲基二异氰酸酯

Abstract:

Amphiphilic functionalization of graphene oxide(GO) is of the essence for its biomedical applications. TWEEN 80-grafted GO was synthesized by a two-step approach. In step 1, GO surface was activated with hexamethylene diisocyanate(HMDI) through a reaction between GO carboxyl or hydroxyl groups and isocyanate. HMDI-activated GO was then grafted by TWEEN 80 in step 2, resulting in amphiphilic GO nano-sheets. The fabricated TWEEN 80-grafted GO exhibited excellent dispersibility in various solvents, such as water, chloroform and n-hexane. Furthermore, PC12 and HeLa cells were cultured on TWEEN 80-grafted GO film to assess its cytotoxicity. Cell morphology analysis and cell viability assay(Calcein AM &EthD-1 staining and MTT assay) indicated that the as-synthesized amphiphilic GO had good biocompatibility.

Key words: Amphiphilic graphene oxide, Biocompatibility, TWEEN 80, Hexamethylene diisocyanate

中图分类号: 

TrendMD: