时间分辨光谱技术研究亚甲基蓝与小牛胸腺DNA的相互作用

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (8): 1854.

时间分辨光谱技术研究亚甲基蓝与小牛胸腺DNA的相互作用

刘涛,张崇磊,陈平,汤国庆,林列

南开大学现代光学研究所, 光电信息技术科学教育部重点实验室, 天津 300071

收稿日期:2010-09-14 修回日期:2010-11-26 出版日期:2011-08-10 发布日期:2011-07-19
通讯作者: 林列 E-mail:linlie@nankai.edu.cn
基金资助:
国家自然科学基金(批准号: 60508004, 60778043)和教育部博士学科点基金(批准号: 20060055024)资助.

Interaction Between Methylene Blue and ctDNA by Time-resolved Spectroscopy

LIU Tao, ZHANG Chong-Lei, CHEN Ping, TANG Guo-Qing, LIN Lie*

Key Laboratory of Optoelectronic Information Science and Technology, Ministry of Education, Institute of Modern Optics, Nankai University, Tianjin 300071, China

Received:2010-09-14 Revised:2010-11-26 Online:2011-08-10 Published:2011-07-19
Contact: LIN Lie E-mail:linlie@nankai.edu.cn
Supported by:
国家自然科学基金(批准号: 60508004, 60778043)和教育部博士学科点基金(批准号: 20060055024)资助.

摘要/Abstract

摘要： 采用时间分辨光谱技术研究了亚甲蓝与小牛胸腺DNA（MB-ctDNA）重水混合体系中MB敏化单态氧（1O2）动力学过程，以此进一步探讨MB与DNA的相互作用。结果表明显示，低浓度ctDNA和高浓度ctDNA的单态氧磷光动力学曲线有着明显不同，这些差异被归结为MB与DNA间结合方式和作用机制的改变。在低浓度ctDNA条件下，MB分子和ctDNA之间形成离子型结合物，MB的吸收带出现显著的减色效应，敏化1O2产量随DNA浓度增加而急剧下降，但ctDNA与1O2没有发生明显的相互作用；而在高浓度DNA时，MB分子和ctDNA之间的相互作用方式转变为以嵌入式结合为主，激发态MB与ctDNA间的能量转移和介质的粘度效应，改变了1O2的动力学特性，大大降低了光敏剂MB敏化1O2的产量，但1O2不为ctDNA所猝灭。这些结果阐明，在MB与DNA的混合体系中，敏化单态氧损坏DNA的Ⅱ型反应不是主要的PDT作用机制。

关键词: 时间分辨光谱; , 单态氧动力学; , 亚甲基蓝; , 小牛胸腺DNA

Abstract: In this paper, the singlet oxygen（1O2） kinetics process of MB-ctDNA mixed solutions in D2O was studied by time-resolved spectroscopy, which helped to further investigate the interaction of MB and DNA. The results showed that 1O2 phosphorescence kinetic curves of MB-ctDNA solutions at low concentrations ctDNA and high concentrations ctDNA had obvious differences, these differences were ascribed to change of MB and DNA binding mode and interaction mechanism. At low ctDNA concentration, ionic conjugate formed between MB molecules and ctDNA, significant hypochromic effect was observed in MB absorption band, and 1O2 production dropped sharply with ctDNA concentration increasing, but there were no obvious interaction between ctDNA and 1O2; at high ctDNA concentration, interaction mode between MB molecules and ctDNA changed to intercalative binding, energy transfer between the excited state MB molecules and ctDNA as well as the effect of medium viscosity changed property of the 1O2 dynamics, which greatly decreased the production of 1O2, but ctDNA did not quench the 1O2 apparently. These results indicated that type II photosensitization of 1O2 damaging DNA was not major mechanism of PDT action in MB-DNA solutions.

Key words: Time-resolved spectroscopy; , Singlet oxygen kinetics; , Methylene blue; , ctDNA

TrendMD:

刘涛张崇磊陈平汤国庆林列. 时间分辨光谱技术研究亚甲基蓝与小牛胸腺DNA的相互作用. 高等学校化学学报, 2011, 32(8): 1854.

LIU Tao, ZHANG Chong-Lei, CHEN Ping, TANG Guo-Qing, LIN Lie*. Interaction Between Methylene Blue and ctDNA by Time-resolved Spectroscopy. Chem. J. Chinese Universities, 2011, 32(8): 1854.

参考文献

[1] Ochsner, M. Journal of Photochemistry and Photobiology B: Biology, [J], 1997. 39(1): p. 1-18. [2] Dolmans, D.E.J.G.J., D. Fukumura and R.K. Jain. Nat Rev Cancer, [J], 2003. 3(5): p. 380-387. [3] Wilson, B.C. and M.S. Patterson. Physics in Medicine and Biology, [J], 2008. 53(9): p. R61-R109. [4] Li, W.-Y., J.-G. Xu, X.-Q. Guo, et al. Analytical Letters, [J], 1997. 30(3): p. 527 - 536. [5] OhUigin, C., D.J. McConnell, J.M. Kelly, et al. Nucl. Acids Res., [J], 1987. 15(18): p. 7411-7427. [6] Tuite, E. and B. Norden. Journal of the American Chemical Society, [J], 1994. 116(17): p. 7548-7556. [7] Zhao, G.-C., J.-J. Zhu, J.-J. Zhang, et al. Analytica Chimica Acta, [J], 1999. 394(2-3): p. 337-344. [8] Dalla Via L, M.M.S. Curr Med Chem. , [J], 2001. 8(12): p. 1405-1418. [9] Orth K, R.D., Beck G, Rück A, Beger HG. Langenbecks Arch Surg., [J], 1998. 383(3-4): p. 276-281. [10] Via LD, M.S. Curr Med Chem, [J], 2001. 8(12): p. 1405-1418. [11] FENEG Xi-Zeng(冯喜增), HE Xi-Wen (何锡文) and S.H.-X. (沈含熙). Chem. J. Chinese Universities(高等学校化学学报), [J], 1996. 17(6): p. 878-882. [12] Tardivo, J.P., A. Del Giglio, C.S. de Oliveira, et al. Photodiagnosis and Photodynamic Therapy, [J], 2005. 2(3): p. 175-191. [13] Skovsen, E., J.W. Snyder, J.D.C. Lambert, et al. The Journal of Physical Chemistry B, [J], 2005. 109(18): p. 8570-8573. [14] Ana Jiménez-Banzo, X.R., Peter Kapusta and Santi Nonell. Photochem. Photobiol. Sci., [J], 2008. 7: p. 1003 - 1010. [15] Floyd, R., J. Schneider and D. Dittmer. Antiviral Research, [J], 2004. 61(3): p. 141-151. [16] Wang, Y. and A. Zhou. Journal of Photochemistry and Photobiology A: Chemistry, [J], 2007. 190(1): p. 121-127. [17] Zhang, L.Z. and G.-Q. Tang. Journal of Photochemistry and Photobiology B: Biology, [J], 2004. 74(2-3): p. 119-125. [18] Zhang, G., S. Shuang, C. Dong, et al. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, [J], 2003. 59(13): p. 2935-2941. [19] Long, E.C. and J.K. Barton. Accounts of Chemical Research, [J], 1990. 23(9): p. 271-273. [20] Schweitzer, C. and R. Schmidt. Chemical Reviews, [J], 2003. 103(5): p. 1685-1758. [21] Schmidt, R. Journal of the American Chemical Society, [J], 1989. 111(18): p. 6983-6987. [22] Boon, E.M., N.M. Jackson, M.D. Wightman, et al. The Journal of Physical Chemistry B, [J], 2003. 107(42): p. 11805-11812. [23] Genereux, J.C. and J.K. Barton. Chemical Reviews, [J], 2009. 110(3): p. 1642-1662.