电喷雾质谱半定量方法研究制川乌配伍后的大鼠肠内菌生物转化

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (7): 1526.

电喷雾质谱半定量方法研究制川乌配伍后的大鼠肠内菌生物转化

王曦烨^1,2,皮子凤¹,刘文龙^1,2,宋凤瑞¹,刘志强¹,刘淑莹¹

1. 中国科学院长春应用化学研究所, 长春130022;
2. 中国科学院研究生院, 北京100049

收稿日期:2010-09-13 修回日期:2010-11-09 出版日期:2011-07-10 发布日期:2011-06-02
通讯作者: 刘淑莹 E-mail:syliu19@yahoo.com.cn
基金资助:
国家“九七三”计划中医理论专项基金(批准号: 2006CB504706)和国家自然科学基金(批准号: 81073040)资助.

Biotransformation of Aconitum Alkaloids Before and After the Combination of Radix Aconiti Preparata by Rat Intestinal Flora Using Semiquantitative Analysis Method of Electrospray Ionization Mass Spectrometry

WANG Xi-Ye^1,2, PI Zi-Feng¹, LIU Wen-Long¹, SONG Feng-Rui¹, LIU Zhi-Qiang¹, LIU Shu-Ying^1*

1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. Graduate School of the Chinese Academy of Science, Beijng 100049, China

Received:2010-09-13 Revised:2010-11-09 Online:2011-07-10 Published:2011-06-02
Contact: LIU Shu-Ying E-mail:syliu19@yahoo.com.cn
Supported by:
国家“九七三”计划中医理论专项基金(批准号: 2006CB504706)和国家自然科学基金(批准号: 81073040)资助.

摘要/Abstract

摘要： 利用建立的代谢前后生物碱成分的电喷雾质谱半定量分析方法，通过分析各药对共煎液经大鼠肠内菌群代谢后主要生物碱的含量变化，对制川乌与浙贝母、法半夏、白蔹分别配伍后共煎液中双酯型、单酯型及脂型生物碱的肠内菌生物转化进行了深入研究，研究结果表明，配伍后中药浙贝母增加共煎液中双酯型生物碱含量，法半夏降低共煎液中双酯型生物碱含量，而白蔹对共煎液中双酯型生物碱含量影响不大；在代谢过程中，大鼠肠内菌群能够将复方中双酯型生物碱转化为脂型生物碱，从而达到中药配伍的减毒增效目的。本文通过化学方法和肠内菌代谢研究证明了制川乌配伍及代谢的机理。

关键词: 制川乌, 配伍, 大鼠肠内菌代谢, 半定量电喷雾质谱分析方法

Abstract: The biotransformation of diester-diterpenoid alkaloids, monoester-diterpenoid alkaloids and lipo-alkaloids in the co-decoctions of Chinese medicine pairs was studied by electrospray ionization tandem mass spectrometry, and semiquantitativ ananlysis method of electrospray ionization tandem mass spectrometry was established for analysis of aconitum alkaloids before and after metabolism. The content changes of aconitum alkaloids with was analyzed under rat intestinal bacteria in the metabolic process of the co-decoctions of Radix Aconiti Preparata with Bulbus Fritillariae Thunbergii, Rhizoma Pinelliae Preparatum and Ampelopsis Japonica. The results showed that Bulbus Fritillariae Thunbergii increased the content of diester-diterpenoid alkaloids in co-decoction, Rhizoma Pinelliae Preparatum reduced the content of diester-diterpenoid alkaloids in co-decoction, and Ampelopsis Japonica almost has no effect on the content of diester-diterpenoid alkaloids in co-decoction, after the combination; The rat intestinal bacteria can more reduce the toxicity of diester-diterpenoid alkaloids and increase the content of lipo-alkaloids. The mechanism of combination and metabolism of traditional Chinese medicine was proved by the analysis of the content changes of aconitum alkaloids in the processes of combination and metabolism of Chinese medicine pairs

Key words: Radix Aconiti Preparata, Combination, Metabolism under Rat Intestinal Bacteria, Semiquantitativ ananlysis method of Electrospray ionization mass spectrometry

TrendMD:

王曦烨皮子凤刘文龙宋凤瑞刘志强刘淑莹. 电喷雾质谱半定量方法研究制川乌配伍后的大鼠肠内菌生物转化. 高等学校化学学报, 2011, 32(7): 1526.

WANG Xi-Ye, PI Zi-Feng, LIU Wen-Long, SONG Feng-Rui, LIU Zhi-Qiang, LIU Shu-Ying*. Biotransformation of Aconitum Alkaloids Before and After the Combination of Radix Aconiti Preparata by Rat Intestinal Flora Using Semiquantitative Analysis Method of Electrospray Ionization Mass Spectrometry. Chem. J. Chinese Universities, 2011, 32(7): 1526.

参考文献

[1]National Pharmacopoeia Committee (国家药典委员会)，The People’s Republic of China Pharmacopoeia(中华人民共和国药典) [M]. Beijing: Chemical Industry Press, 2005 Edition. [2]Sun W.X., Song F.R., Cui M., Liu Z.Q., Liu S.Y. Plata Medica[J], 1999,65:432-435. [3]Wang Y., Liu Z.Q., Song F.R., Liu S.Y. Rapid Commun. in Mass Spectrom.[J], 2002,16:2075-2082. [4] Wang Y., Shi L., Song F.R., Liu Z.Q., Liu S.Y. Rapid Commun. in Mass Spectrom.[J], 2003,17:279-284. [5] Xu Q.X., Wang Y., Cui M., Liu Z.Q., Liu S.Y. Analytical Sciences[J], 2003,19 :1599-1601. [6] Wang Yong(王勇), Liu Zhi-Qiang(李志强), Song Feng-Rui(宋凤瑞), Liu Shu-Ying(刘淑莹). Acta Pharmaceutica Sinica(药学学报) [J], 2003,38：451-454. [7] Wang Yong(王勇)，Song Feng-Rui(宋凤瑞), Jin Dong-Ming(金东明)，Liu Zhi-Qiang(李志强), Liu Shu-Ying(刘淑莹). Chem. J. Chinese Universities(高等学校化学学报) [J], 2004，25：85-89. [8] Hattori Masao. Bacillus Bifidus[J]. 1991, 5:27-40. [9] Yang Xiu-Wei(杨秀伟), Hao Mei-Rong(郝美荣), Hattori Masao. Metabolite Analysis for Chemical Constituents of Traditional Chinese Medicines(中药成分代谢分析)[M], Beijing : Chinese Medicinal and Pharmic Science and Technology Press,2003 :144-151. [10] Zhao Yu-Feng(赵宇峰), Song Feng-Rui(宋凤瑞), Yue Hao(越皓), Guo Xin-Hua(国新华), Li Hui-Lin（李惠琳）, Liu Zhi-Qiang（刘志强）, Liu Shu-Ying（刘淑莹）. Chem. J. Chinese Universities(高等学校化学学报)[J], 2007,11:2051-2055. [11] Zhao Yu-Feng (赵宇峰), Song Feng-Rui (宋凤瑞), Guo Xin-Hua(国新华)，Liu Shu-Ying(刘淑莹) Chem. J. Chinese Universities(高等学校化学学报) [J], 2008,1:55-59. [12] Yue Hao(越皓), Pi Zi-Feng(皮子风), Zhao Yu-Feng(赵宇峰),Song Feng-Rui(宋凤瑞), Liu Zhi-Qiang（刘志强）, Liu Shu-Ying（刘淑莹）. Chinese J. Anal Chem(分析化学)[J]. 2007,7:959-963. [13]Kawapinski J.B.G., Molecular Microbiology(分子微生物学)[M], Beijing:Science Press, 1986,171-183.