高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (7): 1512.

• 研究论文 • 上一篇    下一篇

糖肾方对自发性Ⅱ型糖尿病鼠磷脂代谢的影响

朱超1,2, 黄敏1,2, 梁琼麟2, 王义明2, 胡坪3, 李平4, 张浩军4, 路晓光4, 罗国安1,2   

  1. 1. 华东理工大学药学院, 上海200237;
    2. 清华大学化学系, 生命有机磷与化学生物学教育部重点实验室, 北京100084;
    3. 华东理工大学化学与分子工程学院, 上海200237;
    4. 中日友好医院临床医学研究所, 北京100029
  • 收稿日期:2010-08-16 修回日期:2010-11-09 出版日期:2011-07-10 发布日期:2011-06-02
  • 通讯作者: 罗国安 E-mail:luoga@mail.tsinghua.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:   90707045, 20805026, 90917003)和国家“九七三”计划项目(批准号:   2005CB523503, 2007CB714505)资助.

Effect of  Tangshen Formula  on Phospholipids Metabolism of OLETF Rats

ZHU Chao1,2, HUANG Min1,2, LIANG Qiong-Lin2, WANG Yi-Ming2, HU Ping3, LI Ping4, ZHANG Hao-Jun4, LU Xiao-Guang4, LUO Guo-An1,2*   

  1. 1.  School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;
    2.  Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Ministry of Education,  Department of Chemistry, Tsinghua University, Beijing 100084, China; 
    3.  School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China; 
    4.  Institute of Clinical Medical Research, China-Japan Friendship Hospital, Beijing 100029, China
  • Received:2010-08-16 Revised:2010-11-09 Online:2011-07-10 Published:2011-06-02
  • Contact: LUO Guo-An E-mail:luoga@mail.tsinghua.edu.cn
  • Supported by:

    国家自然科学基金(批准号:   90707045, 20805026, 90917003)和国家“九七三”计划项目(批准号:   2005CB523503, 2007CB714505)资助.

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被引次数

7

摘要： 利用正相液相色谱飞行时间质谱建立了大鼠血浆磷脂的轮廓谱。通过对磷脂轮廓谱的PLS-DA判别分析及14种化合物的定量，考察了中药糖肾方对自发性II型糖尿病鼠磷脂代谢的影响，并对比了糖肾方与西药蒙诺在糖尿病发展过程中的不同作用。结果表明：与正常对照组相比，糖尿病鼠的血浆磷脂代谢发生异常。糖肾方能够调节糖尿病鼠的磷脂代谢紊乱，蒙诺则加剧了病鼠的磷脂代谢异常。此外，8种磷脂化合物可能成为糖尿病发展过程中的潜在生物标志物。

关键词: 糖肾方, OLETF大鼠, 磷脂代谢, NPLC-TOF/MS, PLS-DA

Abstract: As one of the most common endocrine diseases, diabetic mellitus (DM) has a very close relationship with phospholipids metabolism. Tangshen Formula (TSF) is an often-used prescription with good efficacy for treating DM and diabetic nephropathy (DN). The aim of this study is to understand the effect of TSF on phospholipids metabolism of DM. In this paper, Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a model of slowly progressive obese type 2 diabetes, were employed. Phospholipids profiling of rat plasma was established by using normal phase liquid chromatography-time of flight mass spectrometry (NPLC-TOF/MS). Partial least-squares discriminant analysis (PLS-DA) and quantification of 14 compounds were applied to evaluate the effect of TSF on phospholipids metabolism of OLETF rats and to discover potential biomarkers. The results showed plasma phospholipids metabolic disorders occurred in OLETF rats. The good effect of TSF on phospholipids metabolism was compared with the adverse impact of Monopril. TSF is able to significantly improve phospholipids metabolism of OLETF rats, whereas Monopril exacerbated disturbance of phospholipids metabolism. According to quantitative results, a total of 8 phospholipids compounds which have significant concentration variation between control group and model group were considered as potential biomarkers of type II diabetes.

Key words: Tangshen Formula, OLETF Rat, Phospholipids Metabolism, NPLC-TOF/MS, PLS-DA

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