高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (7): 1456.

• 研究论文 • 上一篇    下一篇

VB6与不同CopC作用的光谱研究

宋珍, 郑晓艳, 庞尔国, 杨斌盛   

  1. 山西大学分子科学研究所,  化学生物学与分子工程教育部重点实验室,  太原 030006
  • 收稿日期:2010-10-11 修回日期:2010-12-08 出版日期:2011-07-10 发布日期:2011-06-02
  • 通讯作者: 杨斌盛 E-mail:yangbs@sxu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:   20771068 和 20901048)和山西省留学归国基金(批准号:   201013)资助.

Spectral Studies on the Interaction Between VB6 and Various CopC

SONG Zhen, ZHENG Xiao-Yan, PANG Er-Guo, YANG Bin-Sheng*   

  1. Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China
  • Received:2010-10-11 Revised:2010-12-08 Online:2011-07-10 Published:2011-06-02
  • Contact: YANG Bin-Sheng E-mail:yangbs@sxu.edu.cn
  • Supported by:

    国家自然科学基金(批准号:   20771068 和 20901048)和山西省留学归国基金(批准号:   201013)资助.

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摘要： 在室温, pH 7.4, 10mmol?L-1 Hepes缓冲溶液条件下, 通过紫外差光谱，荧光光谱法研究了apoCopC、CuN-CopC与Ag+的结合性质，进一步研究了apoCopC、 CuN-CopC、CopC-AgC 和CuN-CopC-AgC 与 Vitamin B6 作用的超分子行为. 结果表明：在 apoCopC与Ag+剂量比为1:1时，Ag+可以占据apoCopC蛋白C端的Cu+ 结合位点，结合常数为(1.68±0.33)×104mol?L-1. apoCopC结合不同的金属离子后，与Vitamin B6结合位点数明显减少.

Abstract: The interaction between silver ion and apoCopC, CuN-CopC was investigated through different UV and fluorescence spectra in 10 mmol?L-1 Hepes, pH 7.4, at room temperature. Moreover, the supramolecular behavior between apoCopC, CuN-CopC, CopC-AgC , CuN-CopC-AgC and Vitamin B6 was further studied. The results suggested that the silver ion can occupied the Cu+ site in the 1:1 CopC-AgC complex and the binding constant is (1.68±0.33)×104mol?L-1. After binding of metal ions, the number of binding sites between different proteins and Vitamin B6 was reduced.

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