高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (2): 258.

• 研究论文 • 上一篇    下一篇

去势手术对大鼠肾上腺中孕烯醇酮，17α-羟孕烯醇酮和脱氢表雄酮代谢影响

华海茵1,陈君1,范雪梅2,胡明球3,梁琼麟1,那言群3,王义明1,罗国安1   

  1. 1. 清华大学化学系, 生命有机磷与化学生物学教育部重点实验室, 北京 100084;
    2. 华东理工大学药学院, 上海 200237;
    3. 北京大学第一医院泌尿外科研究所, 北京 100034
  • 收稿日期:2010-05-31 修回日期:2010-06-29 出版日期:2010-02-10 发布日期:2011-02-23
  • 通讯作者: 罗国安;梁琼麟 E-mail:luoga@mail.tsinghua.edu.cn;liangql@tsinghua.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 30772169, 20805026, 90917003)资助.

Impact of Orchiectomy on the Adrenal Metabolism of Pregnenolone, \%17α\%\|Hydroxypregnenolone and Dehydroepiandrosterone in Rats

HUA Hai-Yin1, CHEN Jun1, FAN Xue-Mei2, HU Ming-Qiu3, LIANG Qiong-Lin1*, NA Yan-Qun3, WANG Yi-Ming1, LUO Guo-An1*   

  1. 1. Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua Unisversity, Beijing 100084, China;
    2. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;
    3. Institute of Urology, First Hospital of Peking University, Beijing 100034, China
  • Received:2010-05-31 Revised:2010-06-29 Online:2010-02-10 Published:2011-02-23
  • Contact: LUO Guo-An;LIANG Qiong-Lin E-mail:luoga@mail.tsinghua.edu.cn;liangql@tsinghua.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 30772169, 20805026, 90917003)资助.

PDF (PC)

摘要： 临床对于前列腺癌的主要治疗方式，雄激素阻断治疗，已被证明能够引起肾上腺中CYP17a1基因表达增强. 由于该基因所编码的酶为肾上腺中肾上腺雄激素合成的关键酶，那么去势后由该酶所催化的甾体激素合成途径中的孕烯醇酮、17a-孕烯醇酮以及脱氢表雄酮的含量应该也会出现相应变化. 本文建立了一种适用于生物组织样品中检测三种激素的GC-MS方法. 并通过去势大鼠模型. 对肾上腺中三种激素的含量进行了定量检测分析. 去势后4周，17a-孕烯醇酮和脱氢表雄酮含量显著升高.  

关键词: 去势手术, 肾上腺；气相色谱质谱联用；17α-羟孕烯醇酮；脱氢表雄酮

Abstract: Androgen deprivation therapy, the main method for clinical prostate cancer, had been proved to be able to promote the expression of CYP17a1 gene in adrenal. As CYP17a1 was the crucial enzyme for the production of 17α-hydroxypregnenolone and dehydroepiandrosterone, we established a GC-MS method to illustrate the change of pregnenolone, 17α-hydroxypregnenolone, and dehydroepiandrosterone after orchiectomy in emasculated rat adrenal. 4 weeks after orchiectomy, there was a significant increase of 17α-hydroxypregnenolone and dehydroepiandrosterone accompanied with the increase of CYP17a1 gene expression 2 weeks after orchiectomy in adrenal.

Key words: orchiectomy, adrenal； gas chromatography with mass spectrometry； 17α-Hydroxypregnenolone； dehydroepiandrosterone

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