高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (2): 252.

• 研究论文 • 上一篇    下一篇

Ag(I)-呋塞米相互作用的共振瑞利散射、二级散射和倍频散射光谱及其分析应用

李翠侠,刘绍璞,刘忠芳,胡小莉   

  1. 西南大学化学化工学院, 发光与实时分析教育部重点实验室, 重庆 400715
  • 收稿日期:2010-06-17 修回日期:2010-08-10 出版日期:2010-02-10 发布日期:2011-02-23
  • 通讯作者: 胡小莉 E-mail:xiaolihu@swu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20875078)和发光与实时分析重庆市重点实验室基金(批准号: 2006CA8006)资助.

Resonance Rayleigh Scattering, Second-order Scattering and Frequency Doubling Scattering Spectra of Interaction of Furosemide with Ag(Ⅰ) and Their Analytical Applications

LI Cui-Xia, LIU Shao-Pu, LIU Zhong-Fang, HU Xiao-Li*   

  1. Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China
  • Received:2010-06-17 Revised:2010-08-10 Online:2010-02-10 Published:2011-02-23
  • Contact: HU Xiao-Li E-mail:xiaolihu@swu.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20875078)和发光与实时分析重庆市重点实验室基金(批准号: 2006CA8006)资助.

摘要: 在pH 3.5的HAc-NaAc介质中,呋塞米(FUR)与Ag(I)形成1:1的螯合物,引起共振瑞利散射(RRS)、二级散射(SOS)和倍频散射(FDS)光谱显著增强,其最大RRS,SOS和FDS波长分别位于310、584和330 nm. 在一定范围内,三种散射增强(ΔIRRS, ΔISOS和ΔIFDS)均与呋塞米浓度成正比. 方法具有较高灵敏度,RRS,SOS和FDS法对FUR的检出限(3?)分别为1.3 ng/mL、3.0 ng/mL和6.9 ng/mL. 研究了适宜的反应条件和共存物质的影响,表明方法具有良好的选择性. 据此,提出了简便快速、准确、高灵敏测定痕量呋塞米的光散射新方法,适用于片剂和尿样中呋塞米的测定. 文中还对反应机理和散射光谱增强的原因进行了讨论.

关键词: 呋塞米, Ag(I), 共振瑞利散射光谱, 二级散射, 倍频散射

Abstract: In pH 3.5 HAc-NaAc buffer solution, furosemide reacted with Ag (I) to form a 1: 1 chelate. In this case, the intensities of resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering (FDS) spectra were enhanced greatly and the new scattering spectra appeared. The maximum RRS, SOS and FDS wavelength was located at 310, 584, 330 nm, respectively. The intensities of RRS, SOS and FDS were directly proportional to the concentration of furosemide in a certain range and the detection limit was 1.3 ng/mL, 3.0 ng/mL and 6.9 ng/mL, respectively. In addition, the optimum conditions and the influences of coexisting substances were tested by RRS method and the results showed that this method had good selectivity. Therefore, a new simple, rapid, and sensitive light scattering method has been developed for the determination of furosemide in tablets and urine samples. In addition, the reaction mechanism and the reasons for the enhancement of scattering spectra were discussed.had been investigated. In addition, the optimum conditions of these reactions and the influences of coexisting substances were tested by RRS method and the results showed that this method had good selectivity. Therefore, a highly sensitive, simple and quick method has been developed for the determination of furosemide. The method can be applied satisfactorily to the determination of furosemide in tablets and urine samples. In addition, the reaction mechanism and the reasons for the enhancement of scattering spectra were discussed.

Key words: furosemide, Ag (I), resonance rayleigh scattering, second-order scattering, frequency doubling scattering

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