人参与附子、黄连配伍的HPLC-ESI-MS研究及抗氧化活性测定

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (7): 1332.

人参与附子、黄连配伍的HPLC-ESI-MS研究及抗氧化活性测定

杜芹芹^1,2, 张旭¹, 宋凤瑞¹, 刘志强¹, 刘淑莹¹

1. 中国科学院长春应用化学研究所, 长春质谱中心, 长春 130022;
2. 中国科学院研究生院, 北京 100049

收稿日期:2009-12-10 出版日期:2010-07-10 发布日期:2010-07-10
通讯作者: 刘淑莹, 女, 博士, 研究员, 博士生导师, 主要从事质谱分析研究. E-mail: mslab@ciac.jl.cn
基金资助:
国家支撑计划(批准号: 2007BAI38B04-03)和吉林省科技发展重大及重点项目(批准号: 20086043, 20090908)资助.

HPLC-ESI-MS Analysis of Ginsenosides and Antioxidant Activity During Combination of Ginseng with Aconitum Carmichaeli and Coptis Chinensis

DU Qin-Qin^1,2, ZHANG Xu¹, SONG Feng-Rui¹, LIU Zhi-Qiang¹, LIU Shu-Ying^1*

1. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received:2009-12-10 Online:2010-07-10 Published:2010-07-10
Contact: LIU Shu-Ying. E-mail: mslab@ciac.jl.cn
Supported by:
国家支撑计划(批准号: 2007BAI38B04-03)和吉林省科技发展重大及重点项目(批准号: 20086043, 20090908)资助.

摘要/Abstract

摘要：

采用高效液相色谱与电喷雾质谱联用技术(HPLC-ESI-MS)对不同比例的人参与附子或黄连的水煎液、药渣和沉淀进行研究, 共鉴定了8种人参皂苷, 发现加入附子或黄连后, 溶液中人参皂苷含量明显减少, 进一步研究证明附子或黄连中的某些成分阻止了皂苷的溶出; 同时以抗坏血酸作对照, 应用铁离子还原/抗氧化能力测定法(FRAP)测定了人参单煎液及人参与附子、黄连共煎液中正丁醇提取物和水提取物的抗氧化活性. 结果证明, 共煎液中正丁醇提取物的抗氧化活性高于人参单煎液, 同时也高于人参与附子或黄连单煎混合液的抗氧化活性, 但水提取物抗氧化活性的变化规律有所不同, 这可能与煎煮过程中物质之间的相互作用有关.

关键词: 人参; 附子; 黄连; 液-质联用; 抗氧化

Abstract:

Eight ginsenosides in ginseng, uncombined and combined with Aconitum carmichaeli or Coptis chinensis, have been investigated by high performance liquid chromatography(HPLC) and electrospray ionization-mass spectrometry(ESI-MS). The experimental results demonstrated that the contents of ginsenosides decreased in the decoction of ginseng with Aconitum carmichaeli or Coptis chinensis, and the stripping of ginsenosides was stained during the combination. Furthermore, the antioxidant activity of n-butanol extract in compatible decoction was not only better than the single decoction of ginseng, but also higher than the mixture of the single decoction of ginseng, Aconitum carmichaeli and Coptis chinensis, while the variation of water extract was different, which was determined by ferric reducing antioxidant power(FRAP) method. That might be related to the interaction during the decoction process.

Key words: Ginseng; Aconitum carmichaeli; Coptis chinensis; HPLC-ESI-MS; Anti-oxidation

TrendMD:

杜芹芹, 张旭, 宋凤瑞, 刘志强, 刘淑莹. 人参与附子、黄连配伍的HPLC-ESI-MS研究及抗氧化活性测定. 高等学校化学学报, 2010, 31(7): 1332.

DU Qin-Qin, ZHANG Xu, SONG Feng-Rui, LIU Zhi-Qiang, LIU Shu-Ying*. HPLC-ESI-MS Analysis of Ginsenosides and Antioxidant Activity During Combination of Ginseng with Aconitum Carmichaeli and Coptis Chinensis. Chem. J. Chinese Universities, 2010, 31(7): 1332.

参考文献

[1]YE Jin(叶进). Lishizhen Med. Mater. Med. Res.(时珍国医国药)[J], 2006, 17(8): 1558—1559
[2]SUO Zhi-Rong(索志荣), LIU Xiao-Zhen(刘效诊). J. Chin. Mod. Tradit. Chin. Med.(中华现代中医学杂志)[J], 2005, 1(2): 171—173
[3]LI Li(李丽), LIU Zhi-Qiang(刘志强), XU Qing-Xuan(许庆轩), SONG Feng-Rui(宋凤瑞), LIU Shu-Ying(刘淑莹). J. Instrum. Anal.(分析测试学报)[J], 2006, 25(1): 45—48
[4]WU Wei(吴薇), SONG Feng-Rui(宋凤瑞), LIU Zhi-Qiang(刘志强), LIU Shu-Ying(刘淑莹). Chem. J. Chinese Universities(高等学校化学学报)[J], 2005, 26(1): 27—30
[5]XU Qing-Xuan(许庆轩), LIU Zhi-Qiang(刘志强), WANG Yong(王勇), SONG Feng-Rui(宋凤瑞), LIU Chun-Ming(刘春明), LIU Shu-Ying(刘淑莹). Chin. Tradit. Herb. Drugs(中草药)[J], 2005, 36: 36—39
[6]Tsao R., Deng Z. Y.. J. Chromatogr. B[J], 2004, 812: 85—99
[7]Cui M., Song F. R., Zhou Y., Liu Z. Q., Liu S. Y.. Rapid Commun. Mass Spectrom.[J], 2000, 14: 1480—1486
[8]ZHANG Xu(张旭), SONG Feng-Rui(宋凤瑞), LIU Zhi-Qiang(刘志强), LIU Shu-Ying(刘淑莹). Chem. J. Chinese Universities(高等学校化学学报)[J], 2006, 27(9): 1640—1644
[9]Erbo D., Takao Y., Miroshi K., Masao H., Hiroshi W., Hikokichi O.. Nat. Med.[J], 1996, 50(2): 128—133
[10]Hu C., Kitts D. D.. J. Am. Oil Chem. Soc.[J], 2001, 78(3): 249—255
[11]ZHANG Tao(张涛), WANG Gui-Jie(王桂杰), BAI Shu-Ge(白书阁), BAI Jing(白晶), TIAN Li-Ming(田黎明), HU Wei-Xin(胡伟新). Chin. J. Gerontology(中国老年学杂志)[J], 2001, 21(2): 135—136
[12]LIU Gu-Feng(刘古峰), WU Wei-Kang(吴伟康), DUAN Xin-Fen(段新芬), ZHAO Chi(赵迟), TANG Juan(唐娟). Hainan Med. J.(海南医学)[J], 2008, 19(7): 67—69
[13]JIN Xi-Lu(金昔陆), SHAO Yue(邵悦), WANG Ming-Jie(王铭洁), CHEN Li-Juan(陈丽娟), JIN Guo-Zhang(金国章). Acta Pharmacol. Sinica(中国药理学报)[J], 2000, 21(5): 477—480
[14]WU Yu-Juan(吴玉娟), WANG Yi-Ping(王懿萍), JIANG Yan-Wei(姜延伟), JIANG Yi(姜怡). Lishizhen Med. Mater. Med. Res.(时珍国医国药)[J], 2008, 19(8): 1906—1908
[15]Liu F., Ng T. B.. Life. Sci.[J], 2000, 66(8): 725—735