基于锁核酸的高选择性新型分子信标

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (7): 1322.

基于锁核酸的高选择性新型分子信标

王青, 崔亮, 羊小海, 王柯敏, 何磊良

湖南大学化学生物传感与计量学国家重点实验室, 化学化工学院, 生物医学工程中心, 生物纳米与分子工程湖南省重点实验室, 长沙 410082

收稿日期:2009-12-28 出版日期:2010-07-10 发布日期:2010-07-10
通讯作者: 王柯敏, 男, 博士, 教授, 博士生导师, 主要从事纳米及分子水平上的生物分析化学研究. E-mail: kmwang@hnu.cn
基金资助:
国家“八六三”计划项目(批准号: 2007AA022007)、国家自然科学基金(批准号: 90606003, 20805012)、湖南省杰出青年科学基金(批准号: 08JJ1002)和湖南省高等学校青年骨干教师培养对象基金(2009年度)资助.

Novel Molecular Beacon with High Selectivity Based on Locked Nucleic Acids

WANG Qing, CUI Liang, YANG Xiao-Hai, WANG Ke-Min*, HE Lei-Liang

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Biomedical Engineering Center, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China

Received:2009-12-28 Online:2010-07-10 Published:2010-07-10
Contact: WANG Ke-Min. E-mail: kmwang@hnu.cn
Supported by:
国家“八六三”计划项目(批准号: 2007AA022007)、国家自然科学基金(批准号: 90606003, 20805012)、湖南省杰出青年科学基金(批准号: 08JJ1002)和湖南省高等学校青年骨干教师培养对象基金(2009年度)资助.

摘要/Abstract

摘要：

通过在分子信标的错配位点修饰锁核酸, 不仅可有效地改善其单碱基错配识别能力, 还可提高检测灵敏度. 因而有望发展成为一种通用的提高分子信标单碱基错配识别能力的方法.

关键词: 分子信标; 锁核酸; 选择性

Abstract:

A novel kind of molecular beacon which was modified using locked nucleic acids at mismatch sites was reported. Hybridization kinetics of different molecular beacons with cDNA and single-base mismatch DNA, and melting temperature were investigated using fluorescence spectroscopy. The results showed that molecular beacon with only the mismatch site replaced with LNA had the highest single-base mismatch discrimination capability. Such novel molecular beacon could not only improve effectively single-base mismatch recognition ability, but also enhance the sensitivity. Probably, it was resulted by the high-affinity of locked nucleic acid. Therefore, it was expected to develop a common method for improving the single-base mismatch recognition ability.

Key words: Molecular beacon; Locked nucleic acid; Selectivity

TrendMD:

王青, 崔亮, 羊小海, 王柯敏, 何磊良. 基于锁核酸的高选择性新型分子信标. 高等学校化学学报, 2010, 31(7): 1322.

WANG Qing, CUI Liang, YANG Xiao-Hai, WANG Ke-Min*, HE Lei-Liang. Novel Molecular Beacon with High Selectivity Based on Locked Nucleic Acids. Chem. J. Chinese Universities, 2010, 31(7): 1322.

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