新型β-双链桥连双卟啉的合成及光敏活性

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (7): 1309.

新型β-双链桥连双卟啉的合成及光敏活性

黄齐茂, 李志远, 潘威, 潘志权, 邓鹏星, 肖欣

武汉工程大学绿色化工过程省部共建教育部重点实验室, 武汉 430073

收稿日期:2009-11-09 出版日期:2010-07-10 发布日期:2010-07-10
通讯作者: 潘志权, 男, 教授, 博士生导师, 主要从事大环及配位化学研究. E-mail: huangqim@163.com
基金资助:
国家自然科学基金(批准号: 20471045)和湖北省自然科学基金青年杰出人才项目(批准号: 2008CDB072)资助.

Synthesis of Novel β-Double-bridged Porphyrin Dimers and Their Photosensitive Activity Investigation

HUANG Qi-Mao, LI Zhi-Yuan, PAN Wei, PAN Zhi-Quan*, DENG Peng-Xing, XIAO Xin

Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology, Wuhan 430073, China

Received:2009-11-09 Online:2010-07-10 Published:2010-07-10
Contact: PAN Zhi-Quan. E-mail: huangqim@163.com
Supported by:
国家自然科学基金(批准号: 20471045)和湖北省自然科学基金青年杰出人才项目(批准号: 2008CDB072)资助.

摘要/Abstract

摘要：

设计合成了一种β-双链桥连双卟啉及其Cu(Ⅱ), Ni(Ⅱ), Zn(Ⅱ)配合物, 通过¹H NMR, MS, IR, UV和元素分析进行了表征. 以1,3-二苯基异苯并呋喃(DPBF)为猝灭剂测试了β-双链双卟啉产生单线态氧的能力; 通过DNA凝胶电泳比较了其对pBR322质粒DNA的光敏切割效果; 采用紫外-可见光谱滴定法研究了其与小牛胸腺DNA的相互作用. 实验结果表明, 光敏剂β-双链双卟啉具有较强的产生单线态氧能力, β-双链桥连使双卟啉对pBR322质粒DNA具有较好的光敏切割效果, 与DNA有较强的结合能力.

关键词: 双链双卟啉; DNA; 光敏活性

Abstract:

Four novel β-double-bridged porphyrin dimers were designed and synthesized for the purpose of their photosensitivity and structure-activity relationship study. Their structures were characterized by UV, ¹H NMR, IR, MS and elementary analysis. The ability of singlet oxygen production for the porphyrin dimmers under photo irradiation were determined with DPBF as the quencher. Its photosensitive cleavage ability to pBR322 plasmid DNA has been tested by gel electrophoresis. The interaction of the porphyrin dimer with CT DNA was detected by UV-Vis spectroscopy. The results showed that novel β-double-bridged porphyrin dimers exhibit obvious photosensitive cleavage activity on DNA, resulting from its well binding effects, which is better than that of β-single-bridged porphyrin dimer correspondly.

Key words: Double-bridged porphyrin dimer; DNA; Photosensitive activity

TrendMD:

黄齐茂, 李志远, 潘威, 潘志权, 邓鹏星, 肖欣. 新型β-双链桥连双卟啉的合成及光敏活性. 高等学校化学学报, 2010, 31(7): 1309.

HUANG Qi-Mao, LI Zhi-Yuan, PAN Wei, PAN Zhi-Quan*, DENG Peng-Xing, XIAO Xin . Synthesis of Novel β-Double-bridged Porphyrin Dimers and Their Photosensitive Activity Investigation. Chem. J. Chinese Universities, 2010, 31(7): 1309.

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