高等学校化学学报

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (6): 1280.

• 研究论文 • 上一篇    

超支化聚乙烯亚胺-聚天冬氨酸苄酯共聚物的制备及性能

焦永华, 周军, 李妍, 张开, 付昱   

  1. 东北大学理学院, 沈阳 110004
  • 收稿日期:2009-08-24 出版日期:2010-06-10 发布日期:2010-06-10
  • 通讯作者: 付昱, 男, 教授, 主要从事高分子和超分子研究. E-mail: fuyu@mail.neu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20704003)和超分子结构与材料国家重点实验室开放课题(批准号: SKLSSM200906)资助.

Synthesis, Self-assembly and Gene Transfection Hyperbranched Polyethylenimine-poly(β-benzyl-L-aspartate) Copolymer

JIAO Yong-Hua, ZHOU Jun, LI Yan, ZHANG Kai, FU Yu*   

  1. College of Sciences, Northeastern University, Shenyang 110004, China
  • Received:2009-08-24 Online:2010-06-10 Published:2010-06-10
  • Contact: FU Yu. E-mail: fuyu@mail.neu.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20704003)和超分子结构与材料国家重点实验室开放课题(批准号: SKLSSM200906)资助.

PDF (PC)

被引次数

10

摘要：

以超支化聚合物聚乙烯亚胺为引发剂, 天冬氨酸苄酯-NCA为单体, 利用热开环聚合法合成了超支化聚乙烯亚胺-聚天冬氨酸苄酯共聚物(PEI-PBLA). 用核磁共振波谱对PEI-PBLA的化学结构进行了表征. 研究了PEI-PBLA在水溶液及有机溶液中的自组装行为. 以芘为荧光探针测定了共聚物在水中形成胶束的临界胶束浓度. 以甲基橙为客体分子, 研究了共聚物胶束的包覆能力, 结果表明, 共聚物对客体分子的吸附与溶液的pH值有关. 研究了共聚物与质粒DNA的复合发现, PEI-PBLA能够很好地与质粒DNA复合, 并且可以避免DNA酶的降解, 对其起到一定的保护作用. 此聚合物在药物传输、可控释放及基因载体等方面都有着潜在的应用价值.

关键词: 超支化聚乙烯亚胺(PEI); 聚天冬氨酸苄酯; 高分子胶束; 基因载体

Abstract:

A novel amphiphilic and biodegradable copolymer of hyperbranched polyethylenimine-poly(β-benzyl-L-aspartate)(PEI-PBLA) was synthesized by ring opening polymerization of the N-carboxyanhydride of β-benzyl-L-aspartate(BLA-NCA) with hyperbranched polyethylenimine(PEI) as initiator and characterized by 1H NMR. PEI-PBLA copolymers could form micelles in both organic solvent and water. The micelle formation of PEI-PBLA in CH2Cl2 was displayed by extraction of methyl orange indicator from aqueous solutions. As for PEI-PBLA micelle in water, the critical micelle concentration(cmc) was determined by fluorescence probe method and corresponding morphology was observed by TEM. Meanwhile, the complexation of PEI-PBLA with DNA was explored by gel retardation. The studies imply that PEI-PBLA has great potential in many applications, such as drug delivery, gene transfection and so on.

Key words: Hyperbranched polyethylenimine(PEI); Poly(β-benzyl-L-aspartate); Polymer micelle; Gene vector

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