用于细胞核成像的2,7-BHVC双光子荧光探针

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (6): 1075.

用于细胞核成像的2,7-BHVC双光子荧光探针

贾鹏飞¹, 刘恒¹, 张元红², 刘鑫², 赵宁², 于晓强²

1. 兰州大学生命科学学院, 兰州 730000;
2. 山东大学晶体材料国家重点实验室, 济南 250100

收稿日期:2010-01-18 出版日期:2010-06-10 发布日期:2010-06-10
通讯作者: 刘恒, 男, 博士, 副教授, 主要从事细胞生物学研究. E-mail: hengliu@lzu.edu.cn; 于晓强, 男, 博士, 教授, 博士生导师, 主要从事双光子材料研究. E-mail: yuxq@sdu.edu.cn
基金资助:
国家自然科学基金(批准号: 50673053)和教育部博士点基金(批准号: 200807300014)资助.

Novel Two-photon Fluorescent Probe 2,7-BHVC for DNA Imaging

JIA Peng-Fei¹, LIU Heng^1*, ZHANG Yuan-Hong², LIU Xin², ZHAO Ning², YU Xiao-Qiang^2*

1. School of Life Science, Lanzhou University, Lanzhou 730000, China;
2. State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, China

Received:2010-01-18 Online:2010-06-10 Published:2010-06-10
Contact: LIU Heng. E-mail: hengliu@lzu.edu.cn; YU Xiao-Qiang. E-mail: yuxq@sdu.edu.cn
Supported by:
国家自然科学基金(批准号: 50673053)和教育部博士点基金(批准号: 200807300014)资助.

摘要/Abstract

摘要：

分别合成了2,7-双(1-羟乙基-4-乙烯基吡啶) 咔唑碘盐(2,7-BHVC)与3,6-BHVC, 进行了荧光性能和细胞核成像测试, 结果表明, 2,7位的Ф×δ是3,6位的2.8~3.6倍, 染色实验也证实了2,7-BHVC有特定成像细胞核的能力.

关键词: 双光子荧光探针; 双光子荧光成像; DNA

Abstract:

2,7-BHVC, a novel two-photon fluorescent probe for DNA with large two-photon excited fluorescence action cross sections, was synthesized and characterized. It emits weak two-photon fluorescence in aqueous solution while its two-photon fluorescence is strongly restored upon binding to DNA. The two-photon imaging in cell demonstrated that 2,7-BHVC can exactly label nuclear DNA.

Key words: Two-photon fluorescent probe; Two-photon fluorescence imaging; DNA

TrendMD:

贾鹏飞, 刘恒, 张元红, 刘鑫, 赵宁, 于晓强. 用于细胞核成像的2,7-BHVC双光子荧光探针. 高等学校化学学报, 2010, 31(6): 1075.

JIA Peng-Fei, LIU Heng*, ZHANG Yuan-Hong, LIU Xin, ZHAO Ning, YU Xiao-Qiang*. Novel Two-photon Fluorescent Probe 2,7-BHVC for DNA Imaging. Chem. J. Chinese Universities, 2010, 31(6): 1075.

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