高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (4): 649.

• 研究论文 • 上一篇    下一篇

AMS-8-NH2的合成及其对ODN的负载释放

陈敏敏1, 邹永存2, 孟云君1, 张琪1, 张卓琦3, 曹希传1   

  1. 1. 中国矿业大学材料科学与工程学院, 徐州 221008;
    2. 吉林大学无机合成与制备化学国家重点实验室, 长春 130012;
    3. 徐州医学院附属医院心内科, 徐州 221002
  • 收稿日期:2009-08-13 出版日期:2010-04-10 发布日期:2010-04-10
  • 通讯作者: 曹希传, 男, 博士, 教授, 博士生导师, 主要从事无机纳米生物材料研究. E-mail: xichuancao@yahoo.com.cn
  • 基金资助:

    国家自然科学基金(批准号: 30670557)、江苏省自然科学基金(批准号: BK2008132)和中国矿业大学科技基金(批准号: 2006B029)资助.

Synthesis of AMS-8-NH2 and Its Loading-release of Oligodeoxyneucleotide

CHEN Min-Min1, ZOU Yong-Cun2, MENG Yun-Jun1, ZHANG Qi1, ZHANG Zhuo-Qi3, CAO Xi-Chuan1*   

  1. 1. School of Materials Science and Engineering, China University of Mining and Technology, Xuzhou 221008, China;
    2. State Key Laboratory of Inorganic Synthesis & Preparative Chemistry, Jilin University, Changchun 130012, China;
    3. Department of Cardiology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China
  • Received:2009-08-13 Online:2010-04-10 Published:2010-04-10
  • Contact: CAO Xi-Chuan. E-mail: xichuancao@yahoo.com.cn
  • Supported by:

    国家自然科学基金(批准号: 30670557)、江苏省自然科学基金(批准号: BK2008132)和中国矿业大学科技基金(批准号: 2006B029)资助.

摘要:

以阴离子表面活性剂肉豆蔻酰基谷氨酸钠(C14GluA-Na)为模板剂, 3-氨丙基三甲氧基硅烷(APS)为辅助结构导向剂, 合成了介孔材料AMS-8-NH2, 并结合XRD分析、N2吸附/脱附技术及29Si魔角旋转核磁共振(MAS NMR)等技术手段对纳米孔洞材料AMS-8-NH2进行了表征. 结果表明, AMS-8-NH2是高度有序的纳米孔洞材料, 其孔径和比表面积分别为3.2 nm和706 m2/g; 有机胺基团通过化学键连接在材料内表面, 这些基团的覆盖度达到25%. 以AMS-8-NH2为载体, 寡核苷酸(ODN)为模型生物分子研究在不同条件下AMS-8-NH2对ODN的吸附和释放性能, 实验结果表明, 当ODN浓度一定时, 溶液的pH值越低, 对ODN的吸附量越大, pH=4.7时对ODN的最大负载量可达214 mg/g; 在释放过程中, 材料对ODN具有较高的缓释能力, 随环境pH值增加, 释放量增加.

关键词: 介孔材料AMS-8-NH2; 寡核苷酸; 吸附; 释放

Abstract:

Mesoporous silicas of AMS-8-NH2 have been synthesized with the anionic surfactant N-myristoyl-L-glutamic acid sodium(C14 GluA-Na) and 3-amino propyltrimethoxy silane(APS) as co-structure-directing agents(CSDA). The materials were characterized by powder X-ray diffraction(XRD), N2 adsorption/desorption analysis and 29Si magic angle spinning nuclear magnetic resonance(MAS NMR). The results exhibit that the structure of AMS-8-NH2 has a long range order of three-dimensional cage-like connecting aperture with about 3.2 nm pore diameter and 706 m2/g special surface area respectively nano-hole material. Organic amine groups have anchored chemically to the surface of material through chemical bonding, whose coverage has reached 25%. The resulting AMS-8-NH2 materials were used as vehicles and herring sperm oligodeoxyneucleotide(ODN) as a model biological molecule for discussing the loading and release character of ODN on AMS-8-NH2. The loading experiment shows that when the solution concentration is fixed, the higher solution acidity, the higher loading capability and the maximum amount can reach 214 mg/g at pH=4.7. Among the release process, high sustained release effect of ODN on materials is displayed, which is controlled by environmental pH value. The greater the pH value, the higher the release amount.

Key words: Mesoporous silicas of AMS-8-NH2; Oligodeoxyneucleotide(ODN); Adsorption; Release

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