高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (4): 632.

• 研究快报 • 上一篇    下一篇

双光子荧光有机纳米粒子的细胞染色

徐红1, 杨淑敏2, 陈更1, 田雷蕾2, 何凤2, 王广义1, 马於光2   

  1. 1. 吉林大学第一医院, 长春 130021;
    2. 吉林大学超分子结构与材料国家重点实验室, 长春 130012
  • 收稿日期:2009-10-04 出版日期:2010-04-10 发布日期:2010-04-10
  • 通讯作者: 徐红, 女, 在读博士, 教授, 主要从事消化道肿瘤的临床及基础研究. E-mail: chxuhong@163.com; 王广义, 男, 博士, 教授, 博士生导师, 主要从事消化道肿瘤临床研究. E-mail: wgymd@sina.com
  • 基金资助:

    国家自然科学基金(批准号: 20834006)资助.

Organic Two-photon Fluorescence Nanoparticles for Cell Stains

XU Hong1*, YANG Shu-Min2, CHEN Geng1, TIAN Lei-Lei2, HE Feng2, WANG Guang-Yi1*, MA Yu-Guang2   

  1. 1. The First Hospital of Jilin University, Changchun 130021, China;
    2. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
  • Received:2009-10-04 Online:2010-04-10 Published:2010-04-10
  • Contact: XU Hong. E-mail: chxuhong@163.com; WANG Guang-Yi. E-mail: wgymd@sina.com
  • Supported by:

    国家自然科学基金(批准号: 20834006)资助.

摘要:

本文采用具有较大双光子吸收截面的有机分子2,5,2′,5′-(4′-N,N-二苯胺苯乙烯基)联苯(DPA-TSB)(双光子吸收截面δ: 3288 GM, 1 GM=1×10-50 cm4·s·photon-1·molecule-1), 通过再沉淀法制备水相分散的纳米粒子. 研究表明, 这种有机双光子纳米粒子可以有效地富集在细胞质中, 对细胞染色显示出良好的荧光成像能力.

关键词: 双光子荧光; 有机纳米粒子; 细胞染色

Abstract:

Two-photon absorption materials offer great promise as the new generation of fluorescent probes to image and study biological processes. We present water-dispersed two-photon functional organic nanoparticles which have been fabricated via reprecipitation method based on two-photon absorbing molecule 2,5,2′,5′-tetra(4′-N,N-diphenylaminostyryl)biphenyl(DPA-TSB), aiming to increase active dye loading density and enhance sensitive bioimaging in the microscopic level. In vitro confocal microscopy has demonstrated that these nanoparticles are avidly internalized into cells with bright fluorescence. The good cell membrane permeability of two-photon functional DPA-TSB nanoparticles make them use as bioimaging staining agent for the deeper tissue.

Key words: Two-photon fluorescence; Organic nanoparticle; Cell stain

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