i-Motif在分子拥挤条件下的性质

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (2): 309.

i-Motif在分子拥挤条件下的性质

周俊^1,2, 贾国卿^1,2, 冯兆池¹, 李灿¹

1. 中国科学院大连化学物理研究所, 催化基础国家重点实验室, 大连 116023;
2. 中国科学院研究生院, 北京 100049

收稿日期:2009-07-15 出版日期:2010-02-10 发布日期:2010-02-10
通讯作者: 李灿, 男, 博士, 研究员, 博士生导师, 中国科学院院士, 主要从事物理化学、催化和应用光谱研究. E-mail: canli@dicp.ac.cn
基金资助:
国家自然科学基金(批准号: 20673110 和 20621063)资助.

Properties of i-Motif Under Molecular Crowding Conditions

ZHOU Jun^1,2, JIA Guo-Qing^1,2, FENG Zhao-Chi¹, LI Can^1*

1. State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. Graduate School of Chinese Academy of Sciences, Beijing 100049, China

Received:2009-07-15 Online:2010-02-10 Published:2010-02-10
Contact: LI Can. E-mail: canli@dicp.ac.cn
Supported by:
国家自然科学基金(批准号: 20673110 和 20621063)资助.

摘要/Abstract

摘要：

本文用人端粒富含C的序列[C₃TA₂]₃C₃作为模型, 利用惰性分子PEG 200作为模拟体内分子的拥挤试剂, 通过圆二色光谱和紫外吸收光谱研究了i-Motif在分子拥挤条件下的性质. 结果表明, PEG的存在对i-Motif的结构没有明显影响, 但是可以提高i-Motif的热稳定性.

关键词: i-Motif; 分子拥挤; 聚乙二醇

Abstract:

i-Motif is formed from two parallel duplexes by intercalating with each other in an antiparallel orientation and each duplex is held together via hemiprotonated cytosine⁺-cytosine base pairs. However, a cell is crowded with various biomolecules and little is known about the properties of i-Motif under molecular crowding conditions until now. In the present study, we used human telomeric DNA sequence, [C₃TA₂]₃C₃, as a model system to investigate such problem by circular dichroism(CD) and UV absorbance spectroscopy. Based on the CD spectra, we found that there were no changes about the structure of i-Motif in the presence of polyethylene glycol(PEG). CD melting results showed that the thermal stability of i-Motif was increased under molecular crowding conditions compare to that in dilute buffer, which was further demonstrated by UV-melting results. This work suggests that molecular crowding could not affect the structure of i-Motif, which may depend on pH, while could enhance the thermal stability of i-Motif.

Key words: i-Motif; Molecular crowding; Polyethylene glycol(PEG)

TrendMD:

周俊, 贾国卿, 冯兆池, 李灿. i-Motif在分子拥挤条件下的性质. 高等学校化学学报, 2010, 31(2): 309.

ZHOU Jun, JIA Guo-Qing, FENG Zhao-Chi, LI Can*. Properties of i-Motif Under Molecular Crowding Conditions. Chem. J. Chinese Universities, 2010, 31(2): 309.