CdTe量子点-罗丹明6G荧光共振能量转移体系的构建及其应用研究

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (2): 260.

CdTe量子点-罗丹明6G荧光共振能量转移体系的构建及其应用研究

王绪炎, 梁建功, 马金杰, 陈姝含, 韩鹤友

华中农业大学理学院, 农业微生物学国家重点实验室, 武汉 430070

收稿日期:2009-09-27 出版日期:2010-02-10 发布日期:2010-02-10
通讯作者: 韩鹤友, 男, 教授, 博士生导师, 主要从事纳米生物分析领域的研究. E-mail: hyhan@mail.hzau.edu.cn
基金资助:
国家自然科学基金(批准号: 20975042)、转基因科技重大专项基金(批准号: 2009ZX08012-015B)、湖北省自然科学基金重点项目(批准号: 2008CDA080)和湖北省自然科学基金面上项目(批准号: 2008CDB031)资助.

Construction and Application of CdTe Quantum Dots-Rhodamine 6G Fluorescence Resonance Energy Transfer Systems

WANG Xu-Yan, LIANG Jian-Gong, MA Jin-Jie, CHEN Shu-Han, HAN He-You*

College of Science, State Key Laboratroy of Agricatrual Microbiology, Huazhong Agricultural University, Wuhan 430070, China

Received:2009-09-27 Online:2010-02-10 Published:2010-02-10
Contact: HAN He-You. E-mail: hyhan@mail.hzau.edu.cn
Supported by:
国家自然科学基金(批准号: 20975042)、转基因科技重大专项基金(批准号: 2009ZX08012-015B)、湖北省自然科学基金重点项目(批准号: 2008CDA080)及湖北省自然科学基金面上项目(批准号: 2008CDB031)资助.

摘要/Abstract

摘要：

采用巯基化合物修饰的CdTe量子点构建了量子点(供体)-罗丹明6G(受体)荧光共振能量转移体系, 研究了CdTe量子点与牛血清白蛋白(BSA)的相互作用. 结果表明, CdTe量子点与BSA相互作用后提高了CdTe量子点-罗丹明6G 体系的荧光共振能量转移(FRET)效率, 减小了CdTe量子点和罗丹明6G分子间的距离(r), 证实BSA是通过其色氨酸(Trp)残基与CdTe量子点表面金属发生配位作用而直接结合到量子点表面的.

关键词: CdTe量子点; 罗丹明6G; 荧光共振能量转移; 牛血清白蛋白

Abstract:

Two kinds of thiols modified CdTe quantum dots were used to construct the CdTe quantum dots(donor)-Rhodamine 6G(acceptor) fluorescence resonance energy transfer(FRET) systems, which were applied to investigate the interaction mechanism between CdTe quantum dots and bovine serum albumin(BSA). The results showed that the energy transfer efficiency of the CdTe quantum dots-rhodamine 6G FRET systems were improved, and the distance between CdTe quantum dots and rhodamine 6G(r) were decreased after CdTe quantum dots interacted with BSA, BSA were directly binding to the surface of quantum dot by the coordination between its tryptophan(Trp) residues and the metal.

Key words: CdTe quantum dot; Rhodamine 6G; Fluorescence resonance energy transfer; Bovine serum albumin

TrendMD:

王绪炎, 梁建功, 马金杰, 陈姝含, 韩鹤友. CdTe量子点-罗丹明6G荧光共振能量转移体系的构建及其应用研究. 高等学校化学学报, 2010, 31(2): 260.

WANG Xu-Yan, LIANG Jian-Gong, MA Jin-Jie, CHEN Shu-Han, HAN He-You*. Construction and Application of CdTe Quantum Dots-Rhodamine 6G Fluorescence Resonance Energy Transfer Systems. Chem. J. Chinese Universities, 2010, 31(2): 260.

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