吲哚咪唑基钌(Ⅱ)联吡啶配合物与酵母RNA的相互作用

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (2): 231.

吲哚咪唑基钌(Ⅱ)联吡啶配合物与酵母RNA的相互作用

杨怀霞^1,2, 刘艳菊^1,2, 赵琳¹, 王科志²

1. 河南中医学院药学院, 郑州 450008;
2. 北京师范大学化学学院, 北京 100875

收稿日期:2009-04-27 出版日期:2010-02-10 发布日期:2010-02-10
通讯作者: 王科志, 男, 教授, 主要从事光电和生物活性金属配合物研究. E-mail: kzwang@bnu.edu.cn
基金资助:
国家自然科学基金(批准号: 20771016, 20971016, 90922004)、北京市自然科学基金(批准号: 2072011)、高等学校博士学科点专项科研基金(批准号: 20060027002)和北京师范大学分析测试基金资助.

Interaction of both Indole and Imidazole Group-containing Ruthenium(Ⅱ) Complex with Yeast-RNA

YANG Huai-Xia^1,2, LIU Yan-Ju^1,2, ZHAO Lin¹, WANG Ke-Zhi^2*

1. Pharmacy College, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;
2. College of Chemistry, Beijing Normal University, Beijing 100875, China

Received:2009-04-27 Online:2010-02-10 Published:2010-02-10
Contact: WANG Ke-Zhi. E-mail: kzwang@bnu.edu.cn
Supported by:
国家自然科学基金(批准号: 20771016, 20971016, 90922004)、北京市自然科学基金(批准号: 2072011)、高等学校博士学科点专项科研基金(批准号: 20060027002)和北京师范大学分析测试基金资助.

摘要/Abstract

摘要：

通过紫外-可见光谱和荧光光谱滴定、稳态荧光猝灭和溴化乙啶竞争键合实验研究了Ru(Ⅱ)配合物[Ru(bpy)(H₂iip)₂](ClO₄)₂{bpy=2,2′-联吡啶, H₂iip=2-(吲哚-3-基)-咪唑[4,5-f][1,10]-邻菲罗啉}的酵母RNA键合性质. 结果表明, 二者键合模式为嵌入键合, 其键合常数为7.09×10⁶ L/mol, 比小牛胸腺DNA的键合常数大, 且比同类配合物[Ru(bpy)₂(H₂iip)](ClO₄)₂的酵母RNA键合常数大.

关键词: 钌(Ⅱ); 配合物; 酵母RNA; 吲哚; 联吡啶

Abstract:

The binding of a novel ruthenium(Ⅱ) complex of [Ru(bpy)(H₂iip)₂](ClO₄)₂{bpy=2,2′-bipyridyl, H₂iip=2-(1H-indole-3-yl)-1H-imidazo[4,5-f][1,10]-phenanthroline}, to yeast-RNA was investigated by UV-Visible and emission spectroscopy titrations, steady-state emission quenching and competitive binding with ethidium bromide experiments. The results suggest that the binding of this complex to the RNA be intercalative with a binding constant of 7.09×10⁶ L/mol, which is higher than that to calf thymus DNA and the RNA binding constant of an analogous complex [Ru(bpy)₂(H₂iip)](ClO₄)₂.

Key words: Ruthenium(Ⅱ); Complex; Yeast-RNA; Indole; Bipyridine

TrendMD:

杨怀霞, 刘艳菊, 赵琳, 王科志. 吲哚咪唑基钌(Ⅱ)联吡啶配合物与酵母RNA的相互作用. 高等学校化学学报, 2010, 31(2): 231.

YANG Huai-Xia, LIU Yan-Ju, ZHAO Lin, WANG Ke-Zhi*. Interaction of both Indole and Imidazole Group-containing Ruthenium(Ⅱ) Complex with Yeast-RNA. Chem. J. Chinese Universities, 2010, 31(2): 231.

参考文献

[1]Liu J. G., Zhang Q. L., Shi X. F., et al.. Inorg. Chem.[J], 2001, 40(19): 5045—5050
[2]Ji L. N., Zhou X. H., Liu J. G.. Coord. Chem. Rev.[J], 2001, 216: 513—536
[3]Han M. J., Duan Z. M., Hao Q., et al.. J. Phys. Chem. C[J], 2007, 111(44): 16577—16585
[4]Han M. J., Gao L. H., Lu Y. Y., et al.. J. Phys. Chem. B[J], 2006, 110(5): 2364—2371
[5]HAO Qiang(郝强), HAN Mei-Jiao(韩美娇), ZHENG Shuai-Zhi(郑帅至), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2006, 27(7): 1217—1219
[6]NIE Jing(聂晶), HAN Mei-Jiao(韩美娇), WANG Ke-Zhi(王科志), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2007, 28(10): 1833—1835
[7]XU Hong(徐宏), DENG Hong(邓洪), HU Hong-Yu(胡红雨), et al.. Chem. J. Chinese. Universities(高等学校化学学报)[J], 2003, 24(1): 25—27
[8]JIANG Shang-Da(蒋尚达), WANG Ke-Zhi(王科志), LIU Fu-Rong(刘芙蓉), et al.. Acta Chim. Sinica(化学学报)[J], 2005, 63(8): 783—786
[9]Xu H., Liang Y., Zhang P., et al.. J. Bio. Inorg. Chem.[J], 2005, 10(5): 529—538
[10]Buck D. P., Spillane C. B., Collins J. G., et al.. Mol. BioSyst.[J], 2008, 4(8): 851—854
[11]Spillane C. B., Smith J. A., Buck D. P., et al.. Dalton Trans.[J], 2007, (45): 5290—5296
[12]Xu H., Deng H., Zhang Q. L., et al.. Inorg. Chem. Commun.[J], 2003, (6): 766—768
[13]Mario H., Raed A. Q., Young A. H., et al.. PCT Int. Appl.[J], 2005: 2005047266
[14]YANG Huai-Xia(杨怀霞), LIU Yan-Ju(刘艳菊), ZHAO Lin(赵琳), et al.. Acta Chim. Sinica(化学学报)[J], 2009, 67(4): 352—354
[15]ZHAO Lin(赵琳), WU Bao-Yan(吴宝燕), GAO Li-Hua(高丽华), et al.. Acta Chim. Sinica(化学学报)[J], 2006, 64(13): 1402—1406
[16]ZHAO Lin(赵琳). Studies on Indole- and Imidazole-containing Ruthenium(Ⅱ) Complexes(含吲哚和咪唑基钌配合物的研究)[D], Beijing: Beijing Normal University, 2007
[17]Houten J. V., Watts R. J.. J. Am. Chem. Soc.[J], 1976, 98(16): 4853—4858
[18]Haq I., Lincoln P., Suh D., et al.. J. Am. Chem. Soc.[J], 1995, 117(17): 4788—4796