高等学校化学学报

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (11): 2181.

• 研究论文 • 上一篇    下一篇

基于G-四联体的纳米探针比色检测铅离子

莫志宏, 高应梅, 温志渝, 范艳萍   

  1. 重庆大学化学化工学院, 微系统研究中心, 新型微纳器件与系统技术国防重点学科实验室, 重庆 400044
  • 收稿日期:2010-04-01 出版日期:2010-11-10 发布日期:2010-11-10
  • 通讯作者: 莫志宏, 男, 博士, 教授, 博士生导师, 主要从事生化传感器研究. E-mail: zhihmo@cqu.edu.cn
  • 基金资助:

    国家“八六三”计划项目(批准号: 2007AA042101)和科技部国际科技合作项目(批准号: 2007DFC00040)资助.

Colorimetric Detection of Pb2+ Based on G-Quartet Structure by Nanoprobes

MO Zhi-Hong*, GAO Ying-Mei, WEN Zhi-Yu, FAN Yan-Ping   

  1. National Key Discipline Laboratory of New Micro/Nano Devices and System Technology, Micro-System Research Center, College of Chemistry & Chemical Engineering, Chongqing University, Chongqing 400044, China
  • Received:2010-04-01 Online:2010-11-10 Published:2010-11-10
  • Contact: MO Zhi-Hong. E-mail: zhihmo@cqu.edu.cn
  • Supported by:

    国家“八六三”计划项目(批准号: 2007AA042101)和科技部国际科技合作项目(批准号: 2007DFC00040)资助.

PDF (PC)

被引次数

19

摘要： 基于纳米探针和G-四联体建立了简便快速检测铅离子的方法. 纳米探针采用金纳米粒子自组装修饰富G寡核苷酸制得, 在铅离子存在下, 纳米探针上的富G寡核苷酸形成G-四联体, 导致纳米探针凝聚变色. 在优化条件下, 比色检测铅离子的线性范围为48~480 nmol/L, 检出限为20 nmol/L; 大多数金属离子无明显干扰, 而有明显干扰的汞离子可采用与之特异结合的寡核苷酸有效消除. 将该法成功用于环境水样中铅离子的检测, 重现性(RSD<3.0%)与回收率(98.4%~101.5%)良好.

关键词: 纳米探针, G-四联体, 比色检测, 铅离子

Abstract: A simple and sensitive detection method for lead ions was developed, based on the G-quartet and nanoprobe. The nanoprobe was prepared by modification of gold nanoparticles with G-rich oligonucleotides in a self-assembled way. In the presence of Pb2+, G-quartets are formed by G-rich oligonucleotides on the na-noprobes and result in the aggregation of nanoprobes and a change in color. Under the optimal condition of 200 mmol/L NaCl, 2.0 mmol/L MgCl2 and at 37 ℃, the linear range was 48—480 nmol/L with a detection limit of 20 nmol/L for Pb2+. Most metal ions have no interferences, and the interfering Hg2+ can be effectively eliminated by a specific oligonucleotide. This method was applied for the detection of Pb2+ in environmental water samples, with a good reproducibility(RSD<3.0%) and recovery(98.4%—101.5%).

Key words: Nanoprobe, G-Quartet, Colorimetric detection, Lead ion

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