高等学校化学学报

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (9): 1709.

• 研究论文 • 上一篇    下一篇

莱姆病螺旋体基因型SPR鉴定技术的研究

于东冬1, 刘岩3, 张磊2, 周建光2   

  1. 1. 浙江大学医院内科, 杭州 310027;
    2. 浙江大学分析仪器研究中心, 杭州 310058;
    3. 大连理工大学化工学院化学系, 大连 116024
  • 收稿日期:2009-05-27 出版日期:2009-09-10 发布日期:2009-09-10
  • 通讯作者: 周建光, 男, 博士, 教授, 主要从事分析化学研究. E-mail: jgzhou70@126.com
  • 基金资助:

    浙江省重大科技项目(批准号: 2008C01053-5)资助.

Studies on the Detection of Genotype of Lyme Disease Borrelia Burgdorferi by SPR

YU Dong-Dong1, LIU Yan3, ZHANG Lei2, ZHOU Jian-Guang2*   

  1. 1. Hospital of Zhejiang University, Hangzhou 310027, China;
    2. Research Center for Analytical Instrumentation, Zhejiang University, Hangzhou 310058, China;
    3. Department of Chemistry, College of Chemical Engineering, Dalian University of Technology, Dalian 116024, China
  • Received:2009-05-27 Online:2009-09-10 Published:2009-09-10
  • Contact: ZHOU Jian-Guang. E-mail: jgzhou70@126.com
  • Supported by:

    浙江省重大科技项目(批准号: 2008C01053-5)资助.

PDF (PC)

被引次数

4

摘要：

采用自行设计组装的一套新型表面等离子体子共振(SPR)传感器, 并通过测量计算免疫反应动力学参数——解离常数KD, 对吉林地区常见的莱姆病螺旋体基因型(B.afzelii型和B.garinii型)进行了鉴定研究. 根据实验动物和实际病患血清的鉴定结果可以初步证明, 采用波长型SPR传感器鉴定莱姆病螺旋体基因型具有操作简单、节省时间以及仪器易于小型化、便于推广等特点.

关键词: 表面等离子体共振; 莱姆病; 螺旋体基因型

Abstract:

The genotypes(B.afzelii and B.garinii) of Lyme disease Borrelia burgdorferi were detected successfully by surface plasmon resonance(SPR) sensor. The genotypes of Borrelia burgdorferi were confirmed by the dissociation constant(KD), which calculated from the kinetic curves of immuoreaction between Ag and Ab at different concentrations. This method was simple, less time consumption and accurate to identify genotypes of Borrelia burgdorferi in serum of patients.

Key words: Surface plasmon resonance(SPR); Lyme disease; Borrelia burgdorferi genotype

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