高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (7): 1353.

• 研究论文 • 上一篇    下一篇

新型兼具GPX, SOD, CAT活性的水杨醛Schiff碱衍生物的合成

李唤民1, 高楠2, 刘磊2, 申斯乐1, 罗贵民2   

  1. 1. 吉林大学生命科学学院, 长春 130012;
    2. 吉林大学分子酶学工程教育部重点实验室, 长春 130021
  • 收稿日期:2008-11-06 出版日期:2009-07-10 发布日期:2009-07-10
  • 通讯作者: 罗贵民, 男, 教授, 博士生导师, 主要从事人工酶的研究. E-mail: luogm@jlu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20572035)资助.

Novel Trifunctional Salicylaldehyde Schiff Base Derivative with GPX, SOD and CAT Activities

LI Huan-Min1, GAO Nan2, LIU Lei2, SHEN Si-Le1, LUO Gui-Min2*   

  1. 1. College of Life Science, Jilin University, Changchun 130012, China;
    2. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130021, China
  • Received:2008-11-06 Online:2009-07-10 Published:2009-07-10
  • Contact: LUO Gui-Min. E-mail: luogm@jlu.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20572035)资助.

摘要:

以5-磺基水杨醛为母体, 经氯甲基化和硒化反应引入催化基团—SeH, 此化合物经空气氧化、Schiff碱反应以及锰螯合反应, 最终得到一种新型的水杨醛Schiff碱类谷胱甘肽过氧化物酶(GPX)模拟物. 测定了该模拟物的红外光谱、核磁共振谱、质谱和抗氧化能力. 此模拟物同时兼具超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性, 水溶性好, 保护线粒体免遭氧化损伤能力强, 具有作为药物前体的潜力.

关键词: 谷胱甘肽过氧化物酶; 水杨醛Schiff碱; 硒; 超氧化物歧化酶; 过氧化氢酶

Abstract:

Glutathione peroxidase(GPX) is one of the most crucial antioxidant enzymes in a variety of organisms; it catalyzes the reduction of H2O2 by reduced glutathione(GSH). We used sodium salicylaldehyde-5-sulfonate as the parent of the mimic, then the catalytic group of GPX, —SeH, was introduced to obtain a salicylaldehyde Schiff base compound, which was chelated with Mn and oxidized in air to obtain the final product. Its structure was proved by NMR, MS, IR and its antioxidant ability was tested. The product was demonstrated to have GPX, SOD and CAT activities at the same time, good solubility in water and strong ability to resist oxidative damage. Thus, the trifunctional enzyme mimic possesses a good potential in medicinal application.

Key words: Glutathione peroxidase; Salicylaldehyde Schiff base; Selenium; Superoxide dismutase; Catalase

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