高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (7): 1305.

• 研究论文 • 上一篇    下一篇

共振散射相关光谱一种新的单颗粒探测方法

王康林, 任吉存   

  1. 上海交通大学化学化工学院, 上海 200240
  • 收稿日期:2008-12-29 出版日期:2009-07-10 发布日期:2009-07-10
  • 通讯作者: 任吉存, 男, 教授, 主要从事单分子检测研究. E-mail: jicunren@sjtu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20675052, 20727005)资助.

Resonance Light Scattering Correlation Spectroscopy A New Single Nanoparticles Method

WANG Kang-Lin, REN Ji-Cun*   

  1. College of Chemistry and Chemical Engineering, Shanghai Jiaotong University, Shanghai 200040, China
  • Received:2008-12-29 Online:2009-07-10 Published:2009-07-10
  • Contact: REN Ji-Cun. E-mail: jicunren@sjtu.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20675052, 20727005)资助.

摘要:

基于共焦构型构建了共振散射相关光谱新方法, 阐明了共振散射相关光谱的原理, 并利用纳米金的共振散射特性, 将纳米金标记到生物分子上. 考察了该系统的重现性以及溶液粘度、粒径、浓度和激光能量对金纳米粒子在溶液中扩散行为的影响. 结果表明, 共振散射相关光谱可以替代荧光相关光谱, 应用于生物分析和某些生物系统研究.

关键词: 纳米金; 共振散射相关光谱; 单颗粒检测; 平动扩散

Abstract:

Recently, we developed a new single nanoparticles detection method, called as resonance light scattering correlation spectroscopy(RLSCS). In this article, we first described the principle of RLSCS, and then optimized certain parameters of RLSCS system. Finally, we studied the effects of certain factors such as viscosity of solution, nanoparticle size and laser power on the translational diffusion of gold nanoparticles, and the reproducibility of the setup of RLSCS.

Key words: Gold nanoparticle; Resonance light scattering correlation spectroscopy; Single molecule detection; Translational diffusion

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