高等学校化学学报

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (5): 882.

• 研究论文 • 上一篇    下一篇

表面等离子体子共振传感器用于研究不同材质包裹的磁性纳米粒子性质

陶磊1,2, 金伟1, 张莹1,2, 闫飞2, 王琳琳1,2, 牟颖1,2, 金钦汉1,3   

    1. 浙江大学工业控制技术国家重点实验室, 智能系统与控制研究所, 分析仪器研究中心, 杭州 310058;
    2. 吉林大学分子酶学工程教育部重点实验室, 长春 130021;
    3. 吉林大学化学学院, 长春 130012
  • 收稿日期:2009-01-07 出版日期:2009-05-10 发布日期:2009-05-10
  • 通讯作者: 牟颖, 女, 博士, 教授, 从事整合纳米生物分析技术和生物医学仪器研究, E-mail: ymu100@yahoo.com.cn; 金伟, 男, 博士, 高级工程师, 主要从事分析仪器研究, E-mail: jinweimy@gmail.com
  • 基金资助:

    国家“十一五”科技支撑计划项目(批准号: 2006BAK03A09)、国家“九七三”计划项目(批准号: 2007CB714503)和国家科技基础性工作专项(批准号: 2008IM040800)资助.

Different Materials Coated Magnetic Nanoparticles Studied by Home-made Surface Plasmon Resonance Sensor

TAO Lei1,2, JIN Wei1*, ZHANG Ying1,2, YAN Fei2, WANG Lin-Lin1,2, MU Ying1,2*, JIN Qin-Han1,3   

    1. Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, China;
    2. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130021, China;
    3. College of Chemistry, Jilin University, Changchun 130012, China
  • Received:2009-01-07 Online:2009-05-10 Published:2009-05-10
  • Contact: MU Ying, E-mail: ymu100@yahoo.com.cn; JIN Wei, E-mail: jinweimy@gmail.com

PDF (PC)

被引次数

8

摘要：

利用自行设计组装的以白色发光二极管为光源的表面等离子体子共振传感器实验装置, 检测了不同材质包裹的磁性纳米粒子连接靶向DNA与生物素化DNA探针的结合程度. 结果表明, 与聚苯乙烯磁性微球连接的靶向DNA相比, Fe3O4@SiO2核壳式纳米微球连接的靶向DNA与生物素化的DNA探针结合速率较快, 且其相对标准偏差较小.

关键词: 表面等离子体子共振传感器, 磁性纳米粒子, 生物素化DNA探针, 靶向DNA

Abstract:

By means of surface plasmon resonance(SPR) sensor with a light emission diode(LED) as the excitation source, the association degree of the target DNA coupled with different materials coated magnetic nanoparticles with biotinylated DNA probe was investigated. Compared with that of target DNA coupled with magnetic polystyrene microspheres with biotinylated DNA probe, the association of target DNA conjugated with silica-coated magnetic nanoparticles was faster and the RSD was smaller.

Key words: Surface plasmon resonance sensor, Magnetic nanoparticles, Biotinylated DNA probe, Target DNA

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