槲皮素金属配位分子印迹聚合物的识别性能

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (12): 2514.

槲皮素金属配位分子印迹聚合物的识别性能

范培民, 王兵

教育部中空纤维膜材料与膜过程重点实验室, 天津工业大学环境与化学工程学院, 天津 300160

收稿日期:2009-03-10 出版日期:2009-12-10 发布日期:2009-12-10
通讯作者: 王兵, 男, 博士, 教授, 主要从事分子印迹和分子识别膜材料的研究. E-mail: bingwang666@yahoo.com.cn
基金资助:
天津市自然科学基金(批准号: 05YFJMJC04200)资助.

Molecular Recognition Characteristics of Quercetin′s Metal-complexing Imprinted Polymer

FAN Pei-Min, WANG Bing*

Key Laboratory of Hollow Fiber Membrane Materials and Membrane Process, Ministry of Education, School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160, China

Received:2009-03-10 Online:2009-12-10 Published:2009-12-10
Contact: WANG Bing. E-mail: bingwang666@yahoo.com.cn
Supported by:
天津市自然科学基金(批准号: 05YFJMJC04200)资助.

摘要/Abstract

摘要：

以槲皮素与Zn(Ⅱ)的配合物为模板, 在甲醇溶液中制备金属配位分子印迹聚合物. 通过紫外光谱研究了槲皮素与Zn(Ⅱ)的配位方式及配位比, 验证了槲皮素、Zn(Ⅱ)和4-乙烯基吡啶之间的三元配位作用. 利用红外光谱对产物的结构进行了表征. 用平衡结合实验考察了功能单体及交联剂用量对聚合物吸附性能的影响, 优化了聚合物的反应配比. 同时对系列印迹聚合物的识别体系进行了考察. 结果表明, 槲皮素-Zn(Ⅱ)模板印迹聚合物对槲皮素-Zn(Ⅱ)的配合物表现出明显的吸附选择性和特异性, 对槲皮素结构类似物芦丁和柚皮素的吸附选择性较差, 分离因子分别为3.21和1.91.

关键词: 印迹聚合物; 槲皮素; Zn(Ⅱ); 配位比; 配位方式

Abstract:

Using Zn(Ⅱ)-quercetin complex as template, a metal-complexing template polymer was prepared in methanol by molecular imprinting technique. The coordination mode and complex molar ratio between quercetin and Zn(Ⅱ) were studied in differential UV absorption spectrometry, and the ternary complexation of quercetin, Zn(Ⅱ) and 4-vinylpyridine was verified by similar methods. The molecularly imprinted polymers(MIPs) prepared were analyzed via infrared spectra, and it showed that MIPs had the necessary characteristics of the structure. In order to optimize the reaction ratio of MIPs, the effect of functional monomer and cross-linking agent dosage on the binding capacity of MIPs was studied with equilibrium binding experiments. The selective binding experiments for substrates indicated that compared with quercetin′s (rutin and naringenin), Zn(Ⅱ)-quercetin template polymer exhibited significant adsorption selectivity for Zn(Ⅱ)-quercetin complex. The separation factors were 3.21 and 1.91 respectively.

Key words: Imprinted polymer; Quercetin; Zn(Ⅱ); Complex ratio; Coordination mode

TrendMD:

范培民, 王兵. 槲皮素金属配位分子印迹聚合物的识别性能. 高等学校化学学报, 2009, 30(12): 2514.

FAN Pei-Min, WANG Bing*. Molecular Recognition Characteristics of Quercetin′s Metal-complexing Imprinted Polymer. Chem. J. Chinese Universities, 2009, 30(12): 2514.

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