成骨细胞在表面酰胺化聚乳酸膜上的黏附和增殖行为

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (12): 2491.

成骨细胞在表面酰胺化聚乳酸膜上的黏附和增殖行为

田冶^1,2, 王迎军^1,2, 周长忍³, 曾庆慧³, 谭帼馨⁴

1. 华南理工大学教育部特种功能材料实验室,
2. 材料科学与工程学院, 广州 510640;
3. 暨南大学材料科学与工程系, 广州 510630;
4. 广东工业大学轻工化工学院, 广州 510006

收稿日期:2009-03-27 出版日期:2009-12-10 发布日期:2009-12-10
通讯作者: 周长忍, 男, 教授, 博士生导师, 主要从事生物医用材料方面研究. E-mail: tcrz9@jnu.edu.cn
基金资助:
国家自然科学基金(批准号: 50732003, 50830101, 50803018)和广东省自然科学基金(批准号: 4205786)资助.

Adhesion and Proliferation of Osteoblast on the Amidation Surface of Polylactide Film

TIAN Ye^1,2, WANG Ying-Jun^1,2, ZHOU Chang-Ren^3*, ZENG Qing-Hui³, TAN Guo-Xing⁴

1. Key Laboratory of Special Functional Materials, Ministry of Education,
2. School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China;
3. Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China;
4. Institute of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China

Received:2009-03-27 Online:2009-12-10 Published:2009-12-10
Contact: ZHOU Chang-Ren. E-mail: tcrz9@jnu.edu.cn
Supported by:
国家自然科学基金(批准号: 50732003, 50830101, 50803018)和广东省自然科学基金(批准号: 4205786)资助.

摘要/Abstract

摘要：

利用紫外光接枝聚合丙烯酰胺(Acrylic amide, AAm)获得表面酰胺化的聚乳酸(PLA)膜, 并考察了成骨细胞在酰胺化表面的黏附和增殖行为. 结果表明, 酰胺基的引入改善了PLA膜的表面亲水性, 其表面水接触角由78°减少到56°, 自由能由42.7 mJ/m²增大到51.4 mJ/m²; 与对照组相比, 成骨细胞在改性表面培养3 d后有大量的丝状伪足伸出, 并且较快地进入了细胞分裂期, 表明PLA膜表面的酰胺化能够促进细胞的黏附和增殖.

关键词: 聚乳酸; 酰胺化表面; 接枝聚合; 黏附和增殖

Abstract:

Acrylic amide(AAm) was polymerized onto polylactide(PLA) film via UV irradiation to achieve amidation surface, furthermore the adhesion and proliferation of osteoblast on this surface was investigated. The results show that the hydrophilicity of PLA film is improved by introduction amido: the contact angle decreases from 78° to 56° and the free energy increases from 42.7 mJ/m² to 51.4 mJ/m². Compared with the control, osteoblast spread out more filopodia after 3 d incubation on amidation surface, and it entered cell division phase more quickly, which indicated that the surface amidation could promote the cell′s adhesion and proliferation on the PLA film.

Key words: Polylactide; Amidation surface; Graft polymerization; Adhesion and proliferation

TrendMD:

田冶, 王迎军, 周长忍, 曾庆慧, 谭帼馨. 成骨细胞在表面酰胺化聚乳酸膜上的黏附和增殖行为. 高等学校化学学报, 2009, 30(12): 2491.

TIAN Ye, WANG Ying-Jun, ZHOU Chang-Ren*, ZENG Qing-Hui, TAN Guo-Xing. Adhesion and Proliferation of Osteoblast on the Amidation Surface of Polylactide Film. Chem. J. Chinese Universities, 2009, 30(12): 2491.