巴戟天中一种多糖的分离与结构表征

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (12): 2391.

巴戟天中一种多糖的分离与结构表征

何传波¹, 李琳² , 汤凤霞¹, 熊何健¹

1. 集美大学生物工程学院, 厦门 361021;
2. 华南理工大学轻工与食品学院, 广州 510640

收稿日期:2009-04-09 出版日期:2009-12-10 发布日期:2009-12-10
通讯作者: 何传波, 男, 博士, 讲师, 主要从事多糖物质及其综合利用方面研究. E-mail: hcbcc@sina.com
基金资助:
国家自然科学基金(批准号: 20436020)和广东省科技攻关项目(批准号: 2003C104025)资助.

Isolation and Structure Characterization of Polysaccharide from Morinda officinalis How

HE Chuan-Bo^1*, LI Lin², TANG Feng-Xia¹, XIONG He-Jian¹

1. School of Bioengineering, Jimei University, Xiamen 361021, China;
2. School of Light Chemistry and Food Science, South China University of Technology, Guangzhou 510640, China

Received:2009-04-09 Online:2009-12-10 Published:2009-12-10
Contact: HE Chuan-Bo. E-mail: hcbcc@sina.com
Supported by:
国家自然科学基金(批准号: 20436020)和广东省科技攻关项目(批准号: 2003C104025)资助.

摘要/Abstract

摘要：

以巴戟天的根为原料, 经热水浸提、Sevag法除蛋白、乙醇沉淀和DEAE-Sepharose CL-6B离子交换柱层析, 得到一种水溶性的巴戟天多糖(MOPI-3). 通过UV、IR、NMR、GC-MS、高碘酸氧化、Smith降解和甲基化等物理化学方法对MOPI-3的纯度、理化性质和组成结构进行表征. 结果表明, MOPI-3分子量为36061, 是一种由阿拉伯糖、半乳糖和葡萄糖组成的杂多糖, 以α-1,3-吡喃葡萄糖和α-1,4-吡喃半乳糖为主链, 平均每5个葡萄糖连接一个半乳糖, 每个重复单元具有一个支链, 支链由3个呋喃阿拉伯糖以α-1,3-键型组成, 连接在主链葡萄糖的6位碳上, MOPI-3含有乙酰基, 连接在主链半乳糖的2位碳原子上.

关键词: 巴戟天; 多糖; 分离; 结构表征

Abstract:

Morinda officinalis How is a rare Chinese traditional herbal medicine. A water soluble polysaccharide(MOPI-3) was obtained from Morinda officinalis How, by hot water extraction, deproteination with Sevag method, precipitation with ethanol, and fractionation through DEAE-Sepharose CL-6B ion exchange chromatography. Using UV, IR, NMR, GC-MS, periodate oxidation, Smith degradation and methylation reaction, the primary structure of MOPI-3 was identified. The results show that, MOPI-3 is highly purified with average molecular weight of 35309 determined by high performance gel permeation chromatography. And it is a heteropolysaccharide composed of Ara, Gal and Glc. The backbone of MOPI-3 was linked by α-1,3-pyran-glucose and α-1,4-pyran-galactose, and the molar ratio of glucose to galactose was 5:1. There was one side chain composed by three α-1,3-furan-arabinoses linked onto C6 of glucose on backbone in each repeat unit, and the O-acetyl group was linked onto C2 of galactose.

Key words: Morinda officinalis How; Polysaccharide; Isolation; Structure characterization

TrendMD:

何传波, 李琳 , 汤凤霞, 熊何健. 巴戟天中一种多糖的分离与结构表征. 高等学校化学学报, 2009, 30(12): 2391.

HE Chuan-Bo*, LI Lin, TANG Feng-Xia, XIONG He-Jian. Isolation and Structure Characterization of Polysaccharide from Morinda officinalis How. Chem. J. Chinese Universities, 2009, 30(12): 2391.

参考文献

[1]YAO Hui(姚辉), ZHAO Sheng(赵晟). Plants(植物杂志)[J], 2002, (1): 26—27
[2]WU Yan-Bin(吴岩斌), WU Jin-Zhong(吴锦忠), ZHANG Qiao-Yan(张巧艳), et al.. Pharm. J. Chin. PLA(解放军药学学报)[J], 2009, 25(1): 64—67
[3]YANG Li-Hui(杨黎辉), YAN Fu-Man(闫福曼), CHEN Chao-Feng(陈朝凤), et al.. Acta Chinese Medicine and Pharmacology(中医药学报)[J], 2005, 33(2): 6—8
[4]WANG Yan-Fang(王燕芳), WU Zhao-Hua(吴照华), ZHOU Xin-Yue(周新月), et al.. Acta Botanica Sinica(植物学报)[J], 1986, 28(5): 566—569
[5]ZHAO Yi(赵诣), GUO Su-Hua(郭素华). Strait Pharmaceutical Journal(海峡药学)[J], 2007, 19(2): 59—60
[6]WANG Wei-Ping(王卫平). Lishizhen Medicine and Materia Medica Research(时珍国医国药)[J], 2000, 11(7): 665—666
[7]CUI Cheng-Bin(崔承彬), YANG Ming(杨明), YAO Zhi-Wei(姚志伟), et al.. Chinese Journal of Medicinal Chemistry(中国药物化学杂志)[J], 1995, 5(1): 32—39
[8]Zhong Q. Z., Li Y., Ming Y., et al.. Pharmacology, Biochemistry and Behavior[J], 2002, 72: 39—43
[9]Yun F. L., Zheng H. G., Ming Y., et al.. Life Sciences[J], 2003, 72: 933—942
[10]GU Xiao-Mei(顾笑梅), WU Hou-Ming(吴厚铭), MA Gui-Rong(马桂荣). Chem. J. Chinese Universities(高等学校化学学报)[J], 2004, 25(7): 1288—1290
[11]ZHANG Wei-Jie(张惟杰). Biochemistry Research and Technology of Glycoconjugates, 2nd Edition(糖复合物生化研究技术, 第2版)[M], Hangzhou: Zhejiang University Press, 2003: 37—38; 193—198
[12]Hakomori S. J.. Biochem.[J], 1964, 55: 205—208
[13]Fundin J., Weintraub A., Xu Jian-Guo, et al.. Magnetic Resonance in Chemistry[J], 2003, 41: 202—205
[14]Faber E. F., van den Haak M. J., Kamerling J. P., et al.. Carbohydrate Research[J], 2001, 331: 173—182
[15]SHI Lei(石磊), CHEN Kao-Shan(陈靠山), DONG Qun(董群), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2007, 28(6): 1088—1091
[16]Stefan W., Bjarne H.. Wood Sci. Technol.[J], 2004, 38: 173—179