高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (12): 2387.

• 研究论文 • 上一篇    下一篇

铁氰化钴/树状高分子修饰电极免标记法检测基因突变

朱向彬1, 艾仕云2, 殷焕顺2, 时伟杰2, 刘珍珍2   

  1. 1. 山东农业大学资源与环境学院,
    2. 化学与材料科学学院, 泰安 271018
  • 收稿日期:2008-12-17 出版日期:2009-12-10 发布日期:2009-12-10
  • 通讯作者: 艾仕云, 男, 博士, 教授, 博士生导师, 主要从事功能材料与生物电化学传感器方面的研究. E-mail: ashy@sdau.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20775044)和山东省自然科学基金(批准号: 2006B20)资助.

Label-free Electrochemical Detection of Gene Mutation with the Electrode Modified by Polyamidoamine Dendrimers and Cobalt Hexacyanoferrate

ZHU Xiang-Bin1, AI Shi-Yun2*, YIN Huan-Shun2, SHI Wei-Jie2, LIU Zhen-Zhen2   

  1. 1. College of Resources and Environment,
    2. College of Chemistry and Material Science, Shandong Agricultural University, Tai′an 271018, China
  • Received:2008-12-17 Online:2009-12-10 Published:2009-12-10
  • Contact: AI Shi-Yun. E-mail: ashy@sdau.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20775044)和山东省自然科学基金(批准号: 2006B20)资助.

摘要:

利用铁氰化钴/树状高分子(CoHCF/PAMAM)复合材料修饰玻碳电极(GCE), 制备了免标记检测基因突变的新型DNA电化学传感器. 传感器中树状高分子层明显增加了单链DNA探针的固定量, 铁氰化钴层增大了鸟嘌呤的氧化信号, 该传感器可以灵敏识别单碱基错配的基因序列, 具有良好的选择性和灵敏度. 在7.6×10-11~3.05×10-8 mol/L浓度范围内, 鸟嘌呤(G)的氧化峰电流差值与突变基因浓度呈良好的线性关系, 检出限为1.0×10-11 mol/L(S/N=3).

关键词: 生物传感器; 树状高分子; 免标记法; 杂交; 基因突变

Abstract:

Electrochemical hybridization biosensor for label-free determination of sequence-specific DNA was described. First, cobalt hexacyanoferrate films and poly (amidoamine)(PAMAM) dendrimer(generation 4.0) were modified on the glassy carbon electrode(GCE) sequentially. Then, DNA probes were successfully immobilized on the modified electrode with G4 PAMAM dendrimer acting as the coupling agent. The hybridization events were monitored by differential pulse voltammetry(DPV) measurement based on the oxidation signals of guanine without any external labels. This biosensor exhibited a superior electrochemical stability, selectivity and sensitivity in detection of the oligonucleotide sequence. Under the optimal conditions, the guanine oxidation signal was directly proportional to the mutant gene sequence concentration from 7.6×10-11 to 3.05×10-8 mol/L with a correlation coefficient of 0.9975 and a detection limit of 1.0×10-11 mol/L(S/N=3).

Key words: Biosensor; Polyamidoamine dendrimer; Label-free; Hybridization; Gene mutation

TrendMD: