高等学校化学学报

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (12): 2381.

• 研究论文 • 上一篇    下一篇

基于多孔硅Bragg反射镜的光学免疫检测方法

吕小毅1, 庆格乐图2, 向梅3, 贾振红4, 李锐5, 钟福如3, 李江伟2, 张富春2   

  1. 1. 西安交通大学电子与信息工程学院, 西安 710049;
    2. 新疆大学生命科学与技术学院, 新疆生物资源基因工程重点实验室,
    3. 化学化工学院,
    4. 信息科学与工程学院,
    5. 物理科学与技术学院, 乌鲁木齐 830046
  • 收稿日期:2009-06-22 出版日期:2009-12-10 发布日期:2009-12-10
  • 通讯作者: 贾振红, 男, 博士, 教授, 博士生导师, 主要从事光电器件研究. E-mail: jzhh@xju.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 60968002)、教育部新世纪优秀人才支持计划(批准号: NCET-05-0897)和新疆维吾尔自治区高校科学研究计划(批准号: XJEDU2006I10)资助.

Optical Detection of Immunoreaction Based on the Porous Silicon Bragg Reflector

LÜ Xiao-Yi1, QINGGeLe Tu2, XIANG Mei3, JIA Zhen-Hong4*, LI Rui5, ZHONG Fu-Ru3, LI Jiang-Wei2, ZHANG Fu-Chun2   

  1. 1. School of Electronic and Information Engineer, Xi′an Jiaotong University, Xi′an 710049, China;
    2. Key Laboratory of Xinjiang Biological Resources and Gene Engineering, College of Life Sciences and Technology,
    3. College of Chemistry and Chemical Engineering,
    4. College of Information Science & Engineering,
    5. School of Physics, Xinjiang University, Urumqi 830046, China
  • Received:2009-06-22 Online:2009-12-10 Published:2009-12-10
  • Contact: JIA Zhen-Hong. E-mail: jzhh@xju.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 60968002)、教育部新世纪优秀人才支持计划(批准号: NCET-05-0897)和新疆维吾尔自治区高校科学研究计划(批准号: XJEDU2006I10)资助.

PDF (PC)

被引次数

10

摘要：

通过共价固定方法将羟基红花黄色素A(HSYA)抗血清蛋白固定到多孔硅Bragg反射镜的孔洞中, 定量分析了不同浓度的羟基红花黄色素A人工抗原与特异性抗羟A多克隆抗体反应后多孔硅Bragg反射镜的反射谱峰位的红移情况. 对比研究了固定阴性血清蛋白的多孔硅Bragg反射镜基底在加入抗原后的反射谱峰位变化情况, 结果表明, 基于多孔硅Bragg反射镜的光学免疫检测具有很好的特异性, 且同目前普遍使用的ELISA方法相比, 具有免标记且检测时间短等优异性能, 同时该研究也为开发红花成分快速检测的免标记多孔硅生物传感器奠定了基础.

关键词: 多孔硅Bragg反射镜; 光学检测; 羟基红花黄色素A; 反射光谱

Abstract:

Porous silicon has attracted considerable attention for sensor application due to its remarkable optical properties and the easy fabrication. In this study, an optical immunoassay was developed by the optic reflectance spectroscopy of the porous silicon Bragg reflectors. The artificial immunogen of hydroxysafflor yellow A(HSYA-BSA) and the polyclonal anti-HSYA antibodies were employed as the target and probe, respectively. HSYA is the main chemical component of the flower of the safflower, which is widely used in traditional Chinese medicine for treatment of cerebrovascular and cardiovascular diseases. First, porous silicon Bragg reflector was functionalized using oxidation and silanization. Then, the polyclonal anti-HSYA antibodies were immobilized to the porous silicon Bragg reflector surface used a crosslink method. After the antigen-antibody reaction, the reflection spectrum red shifts and the red shift of spectrum increases with the increase of HSYA-BSA concentration. It is showing the red shifts of the reflectance spectrum as function of HSYA-BSA concentrations. Control experiments were also presented with negative serum and the result suggests that the reflection spectrum red shift is due to selective antigen-antibody binding and this porous silicon Bragg reflector immunosensor possess high specificity. Compared to conventional ELISA, the procedures are label-free and detection time is reduced. Thus, this research lays a foundation of exploiting label-free porous silicon Bragg reflector immunosensor and could be further developed for component detection of safflower of a quick and accurate determination.

Key words: Porous silicon Bragg reflector; Optical detection; Hydroxysafflor yellow A; Reflectances pectrum

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