高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

三维荧光校正法直接测定尿液中的利血平

韩清娟, 吴海龙, 聂瑾芳, 夏阿林, 朱绍华, 张艳, 俞汝勤   

  1. 湖南大学化学化工学院, 化学生物传感与计量学国家重点实验室, 长沙 410082
  • 收稿日期:2006-10-09 修回日期:1900-01-01 出版日期:2007-05-10 发布日期:2007-05-10
  • 通讯作者: 吴海龙

Direct Determination of Reserpine in Urine Using Excitation-Emission Fluorescence Combined with Three-dimensional Chemometric Calibration Methodologies

HAN Qing-Juan, WU Hai-Long*, NIE Jin-Fang, XIA A-Lin, ZHU Shao-Hua, ZHANG Yan, YU Ru-Qin   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
  • Received:2006-10-09 Revised:1900-01-01 Online:2007-05-10 Published:2007-05-10
  • Contact: WU Hai-Long

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被引次数

38

摘要： 利用化学计量学三维数据校正方法中的交替三线性分解算法(ATLD)和自加权交替三线性分解算法(SWATLD), 不经化学分离, 对采用激发-发射矩阵荧光法所得到的三维响应数据阵进行三线性成分分解, 再基于标样已知浓度, 利用简单回归法直接测定尿液中利血平(Reserpine)的含量. 结果表明, 当体系的主要组分数取3时, 两种方法均可迅速、 快捷地得到待测物的浓度, 有效地解决了荧光法定量测定时未知背景及干扰物光谱严重重叠而引起的问题.

关键词: 利血平, 尿液, 激发-发射矩阵荧光, 交替三线性分解(ATLD), 自加权交替三线性分解(SWATLD)

Abstract: The concentration of reserpine in urine was directly and quantitatively measured with the excitation-emission fluorescence(EEM) combined with three-dimensional calibration methodologies. Two calibration methodologies are based on the alternating trilinear decomposition(ATLD) and the self-weighted alternating trilinear decomposition(SWATLD) algorithms, respectively. The satisfactory results of both methods are obtained, when the component number was chosen to be 3(N=3). This experiment is easily carried out without time-consuming and complicated pretreatment. It proved that ATLD and SWATLD based three-dimensional calibration methodologies can be feasible to directly quantify the medical content of reserpine in urine

Key words: Reserpine, Urine, Excitation-emission matrix fluorescence, ATLD, SWATLD

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