高等学校化学学报

高等学校化学学报

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一种新型独特芋螺毒素的分离与结构鉴定

刘尚义, 曹瑛, 赵听友, 代先东, 钟明鼐, 范崇旭, 陈冀胜   

  1. 解放军防化研究院第四研究所, 北京 102205
  • 收稿日期:2005-08-04 修回日期:1900-01-01 出版日期:2006-08-10 发布日期:2006-08-10
  • 通讯作者: 范崇旭

Isolation and Structure Characterization of a New T-Superfamily Conotoxin from Conus Caracteristicus

LIU Shang-Yi, CAO Ying, ZHAO Ting-You, DAI Xian-Dong, ZHONG Ming-Nai, FAN Chong-Xu, CHEN Ji-Sheng   

  1. The 4th Department, Institute of Chemical Defense, PLA, Beijing 102205, China
  • Received:2005-08-04 Revised:1900-01-01 Online:2006-08-10 Published:2006-08-10
  • Contact: FAN Chong-Xu

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被引次数

12

摘要： 从中国南海的一种食虫芋螺独特芋螺(Conus caracteristicus)的毒液中分离得到一种新的芋螺毒素, 对其进行了结构鉴定和化学合成. 结果表明, 这是一种新的T-超家族芋螺毒素.

关键词:

芋螺毒素, T-超家族, 独特芋螺, 结构鉴定

Abstract: Cone snails are tropical marine predatory molluscs, and there is an unprecedented variety of neuropharmacologically active peptide. Over seventy living species of cone snails are distributed in China. The ve-nom of Conus caracteristicus collected from South China Sea was studied in this work. A new conotoxin, cr5a, was isolated from the venom through Sephadex G-25 chromatography and HPLC, and then was identified by amino acid analysis, mass spectrometry, N-terminal sequence. The primary structure of cr5a was CCKFQFLNFCCNE, it shared a conserved T-superfamily of conotoxins arrangement of cysteine residues(CC-CC). Total chemical synthesis of the two possible disulfide linkages of cr5a was achieved, and the result of HPLC co-elution showed that cr5a was a tridecapeptide with a disulfide connectivity of Cys1-Cys10, Cys2-Cys11. So cr5a is a new member of the T-superfamily conotoxins.

Key words: Conotoxins, T-superfamily, Conus caracteristicus, Structure characterization

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