高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

稀土离子(La3+, Gd3+, Yb3+)对线粒体产生活性氧的影响

刘会雪, 杨晓达, 王夔   

  1. 北京大学药学院化学生物学系, 北京 100083
  • 收稿日期:2005-10-25 修回日期:1900-01-01 出版日期:2006-06-10 发布日期:2006-06-10
  • 通讯作者: 杨晓达

Effects of Lanthanide Ions(La3+, Gd3+, Yb3+) on Production of Reactive Oxygen Species in Mitochondria

LIU Hui-Xue, YANG Xiao-Da*, WANG Kui   

  1. Department of Chemical Biology, School of Pharmaceutical Sciences, Peking
    University, Beijing 100083, China
  • Received:2005-10-25 Revised:1900-01-01 Online:2006-06-10 Published:2006-06-10
  • Contact: YANG Xiao-Da

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摘要： 研究了稀土离子对分离的线粒体产生活性氧(ROS)的影响. 采用荧光光度法跟踪线粒体内H2O2生成的动力学, 发现三种稀土离子(La3+, Gd3+, Yb3+)均能降低线粒体H2O2的生成; 用黄嘌呤-黄嘌呤氧化酶体系进一步证明稀土对超氧阴离子(·O-2)存在清除作用, 而对H2O2无清除作用; 测定了稀土对线粒体ROS代谢酶(谷胱甘肽过氧化物酶和超氧化物歧化酶)的活性影响. 结果表明, 三种稀土离子对线粒体谷胱甘肽过氧化物酶的活性基本没有影响, 而Gd3+和Yb3+稀土离子能明显抑制线粒体超氧化物歧化酶的活性.

关键词: 线粒体, 活性氧(ROS)

Abstract: The effects of lanthanides(Ln) on the production of reactive oxygen species(ROS) in isolated mitochondria were investigated by fluorescence assays upon incubation with Ln3+. The results show that Ln3+(La3+, Gd3+, Yb3+) could reduce the rates of overall ROS production. The results obtained from experiments on xanthine/xanthine oxidative enzyme system suggest that Ln3+ ions can reduce the formation of ·O-2, but H2O2 did not react with Ln3+. The influences of Ln3+ on the activities of metabolize enzymes(GSH-Px and Mn-SOD) indicate that Ln3+ could decrease the activity of Mn-SOD, but could not affect the activity of GSH-Px. The effects of Ln3+ on cellular H2O2 level are discussed.

Key words: Lanthanide, Mitochondria, Reactive oxygen species(ROS)

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