高等学校化学学报

高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (5): 859.

• 研究论文 • 上一篇    下一篇

杯菊中的倍半萜内酯

李祖强1, 马国义2, 罗蕾3, 张熹泰2   

  1. 1. 云南大学化学与材料工程学院实验中心, 昆明 650091; 2. 悉尼大学药学系, 澳大利亚;
    3. 云南师范大学化学化工学院, 昆明 650092
  • 收稿日期:2005-06-29 出版日期:2006-05-10 发布日期:2006-05-10
  • 通讯作者: 李祖强(1943年出生)， 男， 教授， 主要从事抗癌活性天然产物研究. E-mail: ZuqiangLi@ynu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号： 29962004)和云南省国际合作项目(批准号： 2001GH21)资助.

Sesquiterpene Lactones from Cyathocline purpurea

LI Zu-Qiang1*, MA Guo-Yi2, LUO Lei3, ZHANG Xi-Tai2   

  1. 1. Experimental Center, College of Chemistry and Material Engineering, Yunnan University, Kunming 650091, China;
    2. Faculty of Pharmacy, the University of Sydney, NSW, Australia;
    3. Chemical & Engineering College, Yunnan Normal University, Kunming 650092, China
  • Received:2005-06-29 Online:2006-05-10 Published:2006-05-10
  • Contact: LI Zu-Qiang, E-mail: ZuqiangLi@ynu.edu.cn

PDF (PC)

被引次数

30

摘要：

摘要杯菊(Cyathocline purpurea)是云南民间民族药, 全株用于治疗各种炎症和肺结核. 采用L1210细胞系对杯菊的粗提物、 分步萃取物的成分进行了细胞毒试验; 用柱层色谱法同步对活性组分进行化学成分分离纯化; 通过理化数据测定及波谱分析鉴定单体结构. 杯菊的氯仿及乙酸乙酯萃取物具有细胞毒活性, 其IC50分别为3.5和2.8 μg/mL. 从活性组分中分离出3种成分, 分别鉴定为Santamarin(1)、 9β-乙酰基广木香内酯(9β-acetoxycostunolide, 2)和9β-乙酰基小白菊内酯(9β-acetoxypathenolide, 3), 其IC50分别为0.41, 0.89, 0.59 μg/mL. 这三个化合物都有较强的抗癌活性, 其中化合物3为新化合物.

关键词: 杯菊; 细胞毒性; 倍半萜内酯; 9β-乙酰基广木香内酯; 9β-乙酰基小白菊内酯

Abstract:

Cyathocline purpurea(Buch-Ham ex D Don) O Ktze is a folk drug used to treat inflammations and pulmonary tuberculosis in Yunnan Province. Cytotoxicity screening of extracts and its fractions from C. purpurea was carried out by L1210 cell. Isolation of chemical constituents by column chromatography was undertaken in step with cytotoxicity test. The structures of constituents were identified by spectroscopic and chemical methods. The chloroform and ethyl acetate extracts possess cytotoxic activity with IC50′s being 3.5 and 2.8 μg/mL, respectively. Three sesquiterpene lactones were established as Santamarin(compound 1), 9β-acetoxycostunolide(compound 2) and 9β-acetoxypathenolide(compound 3). Their IC50 were 0.41, 0.89, 0.59 μg/mL by L1210 cell respectively. The three constituents were obtained for the first time from this plant. They are all anticancer active constituents. The last one is a new compound.

Key words: Cyathocline purpurea; Cytotoxicity; Sesquiterpene lactone; 9β-Acetoxycostunolide; 9β-Acetoxypathenolide

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